"
Template:Team:KULeuven/31 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
| - | # | + | # A miniprep and RD (with EcoRI and PstI) were performed on the four colonies that were ented on Sunday. |
| - | #* | + | #* Nanoprop results |
#* RD results on gel: the right signals were detected | #* RD results on gel: the right signals were detected | ||
{| border ="1" align="center" | {| border ="1" align="center" | ||
| Line 15: | Line 15: | ||
| {{kulpart|BBa_E0240}} || 104,3 || 2,10 || | | {{kulpart|BBa_E0240}} || 104,3 || 2,10 || | ||
|} | |} | ||
| - | # | + | # LigA was plated on LB medium. This will be the "motherplate" from which the glycerol stock and the testing cultures will be taken from. |
| - | # | + | # Electroporation of {{kulpart|BBa_J23101}} in competent cells. It was plated and put overnight in the incubator. |
| - | # | + | # In order to be able to grow vector {{kulpart|pSB3K3}}, which contains the toxic ccdB gene, we need special cells which carry the gyrA462 mutation. This is strain DB3.1 from E.coli. These were plated and put in the incubator overnight. |
Latest revision as of 09:34, 8 September 2009
- A miniprep and RD (with EcoRI and PstI) were performed on the four colonies that were ented on Sunday.
- Nanoprop results
- RD results on gel: the right signals were detected
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| LigA (1) | 110,6 | 1,92 | |
| LigA (2) | 76,7 | 1,99 | |
| LigA (3) | 84,8 | 2,07 | |
| LigA (4) | 31,7 | 2,02 | |
| 104,3 | 2,10 |
- LigA was plated on LB medium. This will be the "motherplate" from which the glycerol stock and the testing cultures will be taken from.
- Electroporation of in competent cells. It was plated and put overnight in the incubator.
- In order to be able to grow vector , which contains the toxic ccdB gene, we need special cells which carry the gyrA462 mutation. This is strain DB3.1 from E.coli. These were plated and put in the incubator overnight.
