Template:Team:KULeuven/6 September 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
 
Line 1: Line 1:
-
* Restriction digest overnight of Sam5 (1,2) and Sam8 (1,2)
+
* Restriction digest overnight of ''sam5'' (1,2) and ''sam8'' (1,2)
* Growth of EF Ligation (woohoo!)
* Growth of EF Ligation (woohoo!)
-
** Transfered 4 colonies from EF ligation to a new plate
+
** Transferred 4 colonies from EF ligation to a new plate
-
** Put the 4 transfered colonies on liquid medium
+
** Put the 4 transferred colonies on liquid medium
-
a miniprep and restrictie digest (ON) on sam5, sam8, ech and fcs was performed on saterday. on sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made.
+
A miniprep and restriction digest (ON) of ''sam5, sam8, ech'' and ''fcs'' was performed on Saturday. On Sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made.

Latest revision as of 12:04, 14 September 2009

  • Restriction digest overnight of sam5 (1,2) and sam8 (1,2)
  • Growth of EF Ligation (woohoo!)
    • Transferred 4 colonies from EF ligation to a new plate
    • Put the 4 transferred colonies on liquid medium

A miniprep and restriction digest (ON) of sam5, sam8, ech and fcs was performed on Saturday. On Sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made.