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Template:Team:KULeuven/7 September 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
(New page: # Restriction digest with EcoRI and PstI on {{kulpart|BBa_E0240}} and the purified pcr product of {{kulpart|BBa_K238013}} in order to get our promotor on intself in the pSB1A2 standard vec...) |
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| - | # Restriction digest with EcoRI and PstI on {{kulpart|BBa_E0240}} and the purified pcr product of {{kulpart|BBa_K238013}} in order to get our promotor on intself in the pSB1A2 standard vector. | + | # Restriction digest with EcoRI and PstI on {{kulpart|BBa_E0240}} and the purified pcr product of {{kulpart|BBa_K238013}} in order to get our promotor on intself in the {{kulpart|pSB1A2}} standard vector. |
# gel electroforesis and extraction of this RD. The concentration of this extraction was too low so no ligation was performed with these samples. | # gel electroforesis and extraction of this RD. The concentration of this extraction was too low so no ligation was performed with these samples. | ||
# extra pcr was done on {{kulpart|BBa_K238013}} with primers 2260 and 2261. | # extra pcr was done on {{kulpart|BBa_K238013}} with primers 2260 and 2261. | ||
# liquid cultures of LigX and of {{kulpart|BBa_J23101}} were made from the 4x plates. | # liquid cultures of LigX and of {{kulpart|BBa_J23101}} were made from the 4x plates. | ||
Revision as of 14:37, 10 September 2009
- Restriction digest with EcoRI and PstI on and the purified pcr product of in order to get our promotor on intself in the standard vector.
- gel electroforesis and extraction of this RD. The concentration of this extraction was too low so no ligation was performed with these samples.
- extra pcr was done on with primers 2260 and 2261.
- liquid cultures of LigX and of were made from the 4x plates.
