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Template:Team:KULeuven/9 September 2009/VanillinProduction
From 2009.igem.org
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* Restriction digest on Terminator | * Restriction digest on Terminator | ||
* Growth on SAMS plates (woohoo!) | * Growth on SAMS plates (woohoo!) | ||
| - | * | + | * Transferred 4 colonies from the SAMS plates (SAMS1, SAMS1 pellet, SAMS2, SAMS2 pellet) to a new plate |
| - | * Gel | + | * Gel electrophoresis of all the 8 EF restriction digests (2 per colony) |
** Cut and purified EF1 and EF3 | ** Cut and purified EF1 and EF3 | ||
{| border ="1" align="center" | {| border ="1" align="center" | ||
Latest revision as of 10:03, 10 September 2009
- Restriction digest on Terminator
- Growth on SAMS plates (woohoo!)
- Transferred 4 colonies from the SAMS plates (SAMS1, SAMS1 pellet, SAMS2, SAMS2 pellet) to a new plate
- Gel electrophoresis of all the 8 EF restriction digests (2 per colony)
- Cut and purified EF1 and EF3
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| EF1 | 5,2 | 1,43 | |
| EF3 | 4,3 | 1,45 |
