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ULB/7 September 2009
From 2009.igem.org
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| + | table.calendar { margin: 0; padding: 10px; } | ||
| + | table.calendar td { margin: 0; padding: 2px; vertical-align: top; } | ||
| + | table.month .heading td { padding:2px; background-color:#3E6DA4; color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } | ||
| + | table.month .dow td { color:#aaa; text-align:center; font-size:110%; } | ||
| + | table.month td.today { background-color:#ddd; } | ||
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| + | #bodyContent table.month a { background:none; padding:0 } | ||
| + | .day-active { color:blue } | ||
| + | .day-empty { color:grey } | ||
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</html> | </html> | ||
Results of ligation: | Results of ligation: | ||
Revision as of 09:01, 15 October 2009
Results of ligation:
- Colonies of ligation with IPTG present a filamentous appearance when we spade with a toothpick.
- Phenotypic tests:
- Spreading bacteria between a glass surface and a sheet of paper: we can feel resistance.
- Comparaison of little balls of spreading bacteria from sticking with those from another transformation: the balls of sticky bacteria have pasted and blanck balls didn't paste.
Extracting of plasmides 9, 10, 5, 6 and 2.
Transformation of 5 ligations carried out on 9/5.
Dubbing of the ligation 1 with IPTG in IPTG medium.
- Results:No fluorescence
Spreading of a large quantity of ligation 1 and 2. Ligation of promoter-RBS-RFP and hfsG-hFSH-terminator.
Following ligations:
- LIG1: Tube 4 (insert) + Tube 5
- Lig2: Tube 5 (insert) + Tube 9
