ULB/7 September 2009

From 2009.igem.org

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== September 7, 2009 ==
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Results of ligation:
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<div style='color:grey;'>
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Résultats de la ligation :
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* Colonies of ligation with IPTG present a filamentous appearance when we spade with a toothpick.
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*Colonies de la ligation avec IPTG présentent un aspect filamenteux lorsqu’’ on pique avec un cure-dent.
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*Tests phénotypiques :
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* Phenotypic tests:
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**Étalement des bactéries sur du verre et on a déposé une feuille de papier dessus : on peut sentir une résistance.
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**Comparaison des billes d’ étalement issues des bactéries collante avec ceux d’ une autres transformation : les billes des bactéries collantes se sont collé et les billes témoins ne sont pas collé.
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** Spreading bacteria between a glass surface and a sheet of paper: we can feel resistance.  
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Extraction des palsmides 9 , 10, 5, 6 et 2.<br/>
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** Comparaison of little balls of spreading bacteria from sticking with those from another transformation: the balls of sticky bacteria have pasted and blanck balls didn't paste.
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Transformation des 5 ligations effectué le 5/9.<br/>
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Répiquage de la ligation 1 avec IPTG dans milieu avec IPTG<br/>
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Extracting of plasmides 9, 10, 5, 6 and 2.
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*Résultats : '''Aucune fluorescence'''<br/>
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Etalement d’ une grande quantité de ligation 1et2<br/>
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Transformation of 5 ligations carried out on 9/5.
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Ligation de promoteur-RBS-RFP et hfsG- hfsH-terminateur<br/>
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Ligations suivante :  
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Dubbing of the ligation 1 with IPTG in IPTG medium.
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#Lig1 : Tube 4 (insert) + Tube 5
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#Lig2 : Tube 5 (insert) + Tube 9
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* Results:'''No fluorescence''' <br/>  
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</div>
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Spreading of a large quantity of ligation 1 and 2.
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Ligation of promoter-RBS-RFP and hfsG-hFSH-terminator.
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Following ligations:  
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# LIG1: Tube 4 (insert) + Tube 5  
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# Lig2: Tube 5 (insert) + Tube 9

Revision as of 21:22, 30 September 2009

Results of ligation:

  • Colonies of ligation with IPTG present a filamentous appearance when we spade with a toothpick.
  • Phenotypic tests:
    • Spreading bacteria between a glass surface and a sheet of paper: we can feel resistance.
    • Comparaison of little balls of spreading bacteria from sticking with those from another transformation: the balls of sticky bacteria have pasted and blanck balls didn't paste.

Extracting of plasmides 9, 10, 5, 6 and 2.

Transformation of 5 ligations carried out on 9/5.

Dubbing of the ligation 1 with IPTG in IPTG medium.

  • Results:No fluorescence

Spreading of a large quantity of ligation 1 and 2. Ligation of promoter-RBS-RFP and hfsG-hFSH-terminator.

Following ligations:

  1. LIG1: Tube 4 (insert) + Tube 5
  2. Lig2: Tube 5 (insert) + Tube 9
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