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Virginia Commonwealth/10 July 2009
From 2009.igem.org
(Difference between revisions)
(New page: ==Friday 10 July 2009== ===Results=== ''Afton and Maria'' *Overnight culture was successful ~~~~ ---- ===Tasks=== ''Afton and Maria'' * Make stocks of parts J23106 and J06702 * Miniprep o...) |
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''Afton and Maria'' | ''Afton and Maria'' | ||
* Dilution of overnight culture | * Dilution of overnight culture | ||
| - | **2 mL of overnight culture was added to 4 mL of LB broth | + | **2 mL of overnight culture was added to 4 mL of LB broth (6 mL) |
***2 dilutions were done for each test tube | ***2 dilutions were done for each test tube | ||
**Cells not used in the MiniPrep will be stored in the -80 degree Celsius freezer | **Cells not used in the MiniPrep will be stored in the -80 degree Celsius freezer | ||
[[User:Trentay|Trentay]] 13:13, 10 July 2009 (UTC) | [[User:Trentay|Trentay]] 13:13, 10 July 2009 (UTC) | ||
Revision as of 13:33, 10 July 2009
Contents |
Friday 10 July 2009
Results
Afton and Maria
- Overnight culture was successful
Trentay 13:13, 10 July 2009 (UTC)
Tasks
Afton and Maria
- Make stocks of parts J23106 and J06702
- Miniprep of parts J23106 and J06702
- Digestion of parts J23106 and J06702
- Electrophoresis test of parts J23106 and J06702
- May Ligate parts with backbones pSB1C3 and pSB4C5 and store DNA
- Write out complete plan for experimentation
- Make plans to MaxiPrep
- Write out a plan to organize ideas
Trentay 13:13, 10 July 2009 (UTC)
Wetlab
Afton and Maria
- Dilution of overnight culture
- 2 mL of overnight culture was added to 4 mL of LB broth (6 mL)
- 2 dilutions were done for each test tube
- Cells not used in the MiniPrep will be stored in the -80 degree Celsius freezer
- 2 mL of overnight culture was added to 4 mL of LB broth (6 mL)
Trentay 13:13, 10 July 2009 (UTC)
