Wiki/Team:Warsaw/primers

From 2009.igem.org

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<h1>Primers</h1>
<h1>Primers</h1>
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<h2>'One-armed bandit' strain preparation</h2>
+
<h2>cloning primers</h2>
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<h4>AID and T7 cloning</h4>
+
<h4>cloning primers</h4>
<pre>
<pre>
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<a name="T7lLinkB">T7lLinkB</a>  5' CGGATCCGGCGGTGGCGGTAGTGGAGGAGGTGGGTCTGGAGGCGGAGGGAACACGATTAACATCGCTAAGAAC 3'
+
<a name="croboxF">croboxF</a>  5' ATCTAGATACCTCTGGCGGTGATACTAGTGT 3'
-
<a name="T7lRBSHi">T7lRBSHi</a>  5' CACAAGCTTAGGAGGTGTACTAGATGAACACGATTAACATCGCTAAG 3'
+
<a name="croboxR">croboxR</a>  5' ACACTAGTATCACCGCCAGAGGTATCTAGAT 3'
-
<a name="T7pXbSal">T7pXbSal</a>  5' TCTGTCGACTCTAGATTACGCGAACGCGAAGTCC 3'
+
<a name="invF">invF</a>  5' ATCTAGATTATATTGACAGCGCACAGAGCGGGAA 3'
-
<a name="AIDlNrH">AIDlNrH</a>    5' CGTAAGCTTAGGAGGTGTAGACCATGGACAGCCTCTTGATG 3'
+
<a name="invR">invR</a>    5' GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT 3'
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<a name="AIDpLinB">AIDpLinB</a>  5' CCACTACCGCCACCGCCGGATCCGCCACCTCCGCTGCCTCCACCACCAAGTCCCAAAGTACGAAATGC 3'
+
<a name="hlyF">hlyF</a>  5' TGTCTAGAATGAAAAAAATAATGCTAGTT  3'
 +
<a name="hlyR">hlyR</a>  5' GACTAGTTTATTCGATTGGATTATCTA  3'
 +
<a name="croF">croF</a>  5' ATCTAGAATGGAACAACGCATAACC  3'
 +
<a name="croR">croR</a>  5' GTACTAGTGGGCTGGAATGTGTAAG  3'
 +
<a name="phoF">phoF</a>  5' ATCTAGAATGATGCGCGTACTGGTTGTAG  3'
 +
<a name="phoR">phoR</a>  5' TTACTAGTGATGCGTGGAAGAACGCACAG  3'
 +
<a name="mgtCF">mgtCF</a>  5' ATCTAGATCCATTTTTTCTGGAAGAATAAG  3'
 +
<a name="mgtCR">mgtCR</a>  5' GTACTAGTCCTCGTCATGTTGTTTTATTTTTT 3'
</pre>
</pre>

Revision as of 10:08, 18 July 2009


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Primers

cloning primers

cloning primers

croboxF   5' ATCTAGATACCTCTGGCGGTGATACTAGTGT  3'
croboxR   5' ACACTAGTATCACCGCCAGAGGTATCTAGAT  3'
invF   5' ATCTAGATTATATTGACAGCGCACAGAGCGGGAA  3'
invR    5' GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT  3'
hlyF   5' TGTCTAGAATGAAAAAAATAATGCTAGTT  3'
hlyR   5' GACTAGTTTATTCGATTGGATTATCTA  3'
croF   5' ATCTAGAATGGAACAACGCATAACC  3'
croR   5' GTACTAGTGGGCTGGAATGTGTAAG  3'
phoF   5' ATCTAGAATGATGCGCGTACTGGTTGTAG  3'
phoR   5' TTACTAGTGATGCGTGGAAGAACGCACAG  3'
mgtCF   5' ATCTAGATCCATTTTTTCTGGAAGAATAAG  3'
mgtCR   5' GTACTAGTCCTCGTCATGTTGTTTTATTTTTT  3'


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