Duke University/17 June 2009

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June 17, 2009

  • E: PCR with Bioplastics #4 as template, using Phusion enzyme protocol (shorter denaturation time, lower annealing temperature), Gel to check PCRs
98°C 15s 1x
98°C 1s
50°C 5s 
72°C 30s 35x
72°C 2m
4°C infinity 1x
  • R: Correct bands
  • C: Cut out bands


  • E: Measured concentration of DNA fragments 3 and 4
  • R: Concentrations high
  • C: Do assembly PCR