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Duke University/17 June 2009
From 2009.igem.org
June 17, 2009
- E: PCR with Bioplastics #4 as template, using Phusion enzyme protocol (shorter denaturation time, lower annealing temperature), Gel to check PCRs
98°C 15s 1x 98°C 1s 50°C 5s 72°C 30s 35x 72°C 2m 4°C infinity 1x
- R: Correct bands
- C: Cut out bands
- E: Measured concentration of DNA fragments 3 and 4
- R: Concentrations high
- C: Do assembly PCR
