EPF-Lausanne/11 September 2009

Wet Lab

O.D. for the Trp K.O. strains

For the competent cells !

After 5p.m. :

JRG1046 : 1.35. JRG465 : 0.85. JW4356-2 : 1.64. JW4356-2 (Kana) : 1.57.

For today we drop the experiment, we are too far from 0.6. We will do it on Monday.

Results of the characterization

RO2-BB #1, all clones were illuminated :

RO2-BB#1 plot

We have induction (repression repressed...) from LovTAP, we have to investigate further to be sure it is what we want.

Tryptophan concentration experiment on RO2 :

RO2#4 TRP plot

All the clones react in the same way, here it is the graph for RO2#4. It seems we have an increase until somewhere between 2ul and 4 ul (in 50 ul of culture, TRP : 4.25 g/L).

RO2#5

RO2#5 plot

RO2#10

RO2#10 plot

RO1-BB didn't respond.

RO2#4, influence of TRP and ATC

Nicolas ce graph n'est pas dans ce que tu nous a envoyé, est-ce que tu peux le rajouter stp ???

Read out 1 characterization

To summarize our results, we did the following graphic :

RO1 final plot

We can say that Read Out 1 is characterized, it shows exactly the answer we would expect : if we add TRP, there is a significant decrease in RFP expression.

People in the lab

Mélanie, Caroline, Basile, Nicolas