Illinois/7 July 2009

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July 7

sRNA Library

We ran two gels with all of our digestion products.

Digest1uiuc.jpg

Lane 1 100bp ladder

    2    ftsZ
    3    ftsZ
    4    oppA
    5    oppA
    6    dppA
    7    dppA
    8    hns
    9    hns
    10   galK

Digest2uiuc.jpg

Lane 1 100bp ladder

    2    galK
    3    dicF
    4    dicF
    5    gcvB
    6    gcvB
    7    rseX
    8    rseX
    9    ryhB
    10   ryhB

The samples were split up into two lanes just in case the wells on the gels didn't overflow.

After the digestion gels, we performed a gel purification and used the nanodrop to check the DNA concentrations for each of the digestion products.

Concentrations: ng/microliter


ftsZ: 21.6 oppA: 14.1 dppA: 15.0 hns: 11.8 galK: 19.0 dicF: 23.0 gcvB: 21.3 rseX: 6.8 ryhB: 7.5


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