User contributions
From 2009.igem.org
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- 18:11, 21 October 2009 (diff | hist) Team:KULeuven/Dear Judges (→Dear Judges)
- 18:11, 21 October 2009 (diff | hist) Team:KULeuven/Dear Judges (→Dear Judges,)
- 22:23, 19 October 2009 (diff | hist) N Template:Team:KULeuven/14 October 2009/BlueLightReceptor (New page: experiment from 07/10 was repeated) (top)
- 22:21, 19 October 2009 (diff | hist) N Template:Team:KULeuven/7 October 2009/BlueLightReceptor (New page: experiment of 24/09 was repeated but the cultures were only grown and lit at 25°C. only the logA were used (in pBR322 vector) because its signal was clearer.)
- 22:19, 19 October 2009 (diff | hist) N Template:Team:KULeuven/6 October 2009/BlueLightReceptor (New page: * the strenght of the promoter was tested following the protocol from the standard promoter measurement kit from iGEM. this can be found on the parts registry. * liquid cultures of blp+gfp...) (top)
- 22:16, 19 October 2009 (diff | hist) N Template:Team:KULeuven/5 October 2009/BlueLightReceptor (New page: liquid cultures of top10 I20260 standard promoter and our own construct (blp+gfp) were made.) (top)
- 22:15, 19 October 2009 (diff | hist) Team:KULeuven/Notebook/Blue Light Receptor (top)
- 22:11, 19 October 2009 (diff | hist) N Template:Team:KULeuven/29 September 2009/BlueLightReceptor (New page: we redid the experiment from 24/09 but only grew cultures at 25°C. we did split up the cultures into two groups, 16°c and 25°C and each group had some cultures that were lit and some da...) (top)
- 22:06, 19 October 2009 (diff | hist) N Template:Team:KULeuven/28 September 2009/BlueLightReceptor (New page: re enting of the cultures made on sunday (27/09)) (top)
- 22:02, 19 October 2009 (diff | hist) Team:KULeuven/Notebook/Blue Light Receptor
- 21:43, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 21:42, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 09:27, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 09:26, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 09:23, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 09:21, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 09:20, 19 October 2009 (diff | hist) N File:Light-dark.png (top)
- 09:19, 19 October 2009 (diff | hist) N File:Weakness.png (top)
- 08:10, 16 October 2009 (diff | hist) Team:KULeuven/Medal Checklist (→Gold medal requirements)
- 09:12, 14 October 2009 (diff | hist) Team:KULeuven/Medal Checklist (→Bronze medal requirements)
- 10:27, 28 September 2009 (diff | hist) N Template:Team:KULeuven/27 September 2009/BlueLightReceptor (New page: # enting of ligA, LikA and LogA for measurementof the promoter activity when grown at 25°C) (top)
- 10:25, 28 September 2009 (diff | hist) Template:Team:KULeuven/25 September 2009/BlueLightReceptor (top)
- 10:23, 28 September 2009 (diff | hist) N Template:Team:KULeuven/25 September 2009/BlueLightReceptor (New page: # measurement of the liquid culuters of 24/09: * at first glance it seemed that there was indeed repression of the promoter on 25°. further conclusions from the results could however not ...)
- 10:20, 28 September 2009 (diff | hist) N Template:Team:KULeuven/24 September 2009/BlueLightReceptor (New page: #following the conclusions made on 23/09 a new experiment was set up: * liquid cultures were ented (and reented in the evening) and grown on two different temperatures: 37° and 25°. * f...) (top)
- 10:12, 28 September 2009 (diff | hist) N Template:Team:KULeuven/23 September 2009/BlueLightReceptor (New page: # measurements of the liquid cultures from 22/09 were made. the empty top10, and standard promoter I20260 were measured. then, at different times likA were measured. we concluded the foll...) (top)
- 15:16, 27 September 2009 (diff | hist) N Template:Team:KULeuven/22 September 2009/BlueLightReceptor (New page: # liquid cultures of LikA, {{kulpart|BBa_I20260}} and the empty top10 cells were reented. 100µl was taken and put in new medium to get a dilution of 1/50. they grew during the day. in the...) (top)
- 15:22, 21 September 2009 (diff | hist) Template:Team:KULeuven/21 September 2009/BlueLightReceptor (top)
- 13:21, 21 September 2009 (diff | hist) Template:Team:KULeuven/21 September 2009/BlueLightReceptor
- 10:11, 21 September 2009 (diff | hist) N Template:Team:KULeuven/21 September 2009/BlueLightReceptor (New page: # a new restriction digest on logA (1&2) was done. # the reenting of likA was succesfull and the liquid culture was miniprepped. likA: 179,3 ng/µl # a glycerol stock of ligY was made and ...)
- 10:06, 21 September 2009 (diff | hist) Template:Team:KULeuven/4 September 2009/BlueLightReceptor (top)
- 10:05, 21 September 2009 (diff | hist) N Template:Team:KULeuven/6 September 2009/BlueLightReceptor (New page: # There was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. This was all done in a dark room to avoid activation of th...) (top)
- 09:53, 21 September 2009 (diff | hist) N Template:Team:KULeuven/5 September 2009/BlueLightReceptor (New page: #Electroporation of ligX 1&2 #There was no growth of cells after electroporation with {{kulpart|pSB3K3}}) (top)
- 09:49, 21 September 2009 (diff | hist) Team:KULeuven/Notebook/Blue Light Receptor
- 09:47, 21 September 2009 (diff | hist) Template:Team:KULeuven/20 September 2009/BlueLightReceptor (top)
- 09:46, 21 September 2009 (diff | hist) Template:Team:KULeuven/18 September 2009/BlueLightReceptor (top)
- 09:44, 21 September 2009 (diff | hist) N Template:Team:KULeuven/20 September 2009/BlueLightReceptor (New page: # the elecroporation from saterday was not succesfull for logA but there was a colony on the likA plates. this was reented and a liquid culture was made to miniprepp and check. # the rest...)
- 09:43, 21 September 2009 (diff | hist) N Template:Team:KULeuven/19 September 2009/BlueLightReceptor (New page: # enting of the electroporated logA from 18/09. the enting was done on tc(5) as wel as on ap plates. there should not be any growth on the ap plates since the resistence gene is cut in hal...) (top)
- 09:41, 21 September 2009 (diff | hist) Team:KULeuven/Notebook/Blue Light Receptor
- 09:37, 21 September 2009 (diff | hist) Template:Team:KULeuven/18 September 2009/BlueLightReceptor
- 09:21, 21 September 2009 (diff | hist) Template:Team:KULeuven/17 September 2009/BlueLightReceptor (top)
- 09:18, 21 September 2009 (diff | hist) N Template:Team:KULeuven/18 September 2009/BlueLightReceptor (New page: # gel electrophoresis of the RD of ligY and ligA. all of the inserts were ok and ligA had a right signal. # a motherplate for ligY was made # likA and LogA ligations were electroporated)
- 09:15, 21 September 2009 (diff | hist) N Template:Team:KULeuven/17 September 2009/BlueLightReceptor (New page: # electroporation of logA (ligA in pBR322, a low copy plasmid). # a PCR on pSB3K3 with primers ...... # restriction digest of pSB3K3, pBR322 and ligA with EcoRI and PstI followed by a gel ...)
- 14:39, 16 September 2009 (diff | hist) Template:Team:KULeuven/16 September 2009/BlueLightReceptor (top)
- 14:36, 16 September 2009 (diff | hist) N Template:Team:KULeuven/15 September 2009/BlueLightReceptor (New page: # miniprep of pBR322 (nanodrop: 24,5ng/µl) # restriction digest of pBR322 with EcoRI and PstI # gelelectrophoresis and extraction of pBR322 (nanodrop: 10,8ng/µl) # ligation of pBR322 wit...) (top)
- 14:28, 16 September 2009 (diff | hist) N Template:Team:KULeuven/16 September 2009/BlueLightReceptor (New page: # miniprep of pRB322 (nanodrop: 59,8ng/µl))
- 09:36, 16 September 2009 (diff | hist) N Template:Team:KULeuven/14 September 2009/BlueLightReceptor (New page: # miniprep of ligY colonies. nannodrop: 111 ng/µl and 116 ng/µl. # restriction digest of ligY miniprep # gelelectrophoresis of restricted ligY and compared to ligA. the ligY fragment wa...) (top)
- 14:03, 11 September 2009 (diff | hist) Template:Team:KULeuven/11 September 2009/BlueLightReceptor
- 09:53, 11 September 2009 (diff | hist) Team:KULeuven/Design/Blue Light Receptor
- 09:45, 11 September 2009 (diff | hist) Template:Team:KULeuven/11 September 2009/BlueLightReceptor
- 08:21, 11 September 2009 (diff | hist) Template:Team:KULeuven/8 September 2009/VanillinProduction
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