Team:Paris/13 August 2009

From 2009.igem.org

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August 13th

Lab work

Transformation check

  • Transformation of yesterday (12/08) doesn't work, we have colonies in all the 3 box (2 with inserts and vector and 1 negative control with only vector which normaly should don't have bacteria)
  • maybe it's the ligation which has bad done, we maybe have to increase incubation time.

All Manip

Comming soon

To do list

Matricule TODO
Luc
Romain
Charlotte
Stoff
Chris
Lisa
Caroline
Souf
Vicard
Pierre
Sylvain pSB2K3 Transformation and Minicult , OmpA-Linker standardization (gels, purifs, digestions etc ... or some different PCRs / matrices ), Minipreps, a bit thinking about an enzymatic expression to enhance signal
Guillaume


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