Template:Team:KULeuven/16 September 2009/VanillinProduction
From 2009.igem.org
- Restrictions from yesterday were put on gel (COMT2, 3, SAMS, Promotor)
- SAMS and promotor( J23110??) were gel purified
| part | concentration | 260/280 | 260/230
|
| SAMS | 8,5 | |
|
| Promotor | 4,5 | |
|
- Ligation of SAMS + promotor
- 28 μl of SAMS and 11 μl from promotor, almost 4:1 ratio (although maybe closer to 3:1)
- Electroporation of EFT ligation from yesterday on Km plates (Ter vector has Km resistance, so a closing of EF vector on its own doesn't grow!)
- Miniprep
| part | concentration | 260/280 | 260/230
|
| 1 | 69,2 | 1,99 |
|
| 2 | 34,5 | 2,01 |
|
| 3 | 74,9 | 2,03 |
|
- The liquid culture of SAMS was used for the glycerol stock and send for sequencing
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