RNA - DNA Report

Report time                    2009-08-29 17:57:48                                
Batch name                     C:\Documents and Settings\Ellenor                  
                               Olsson\Desktop\iGEM\Plasmidprep_90829_3.BDN        
Application                    RNA - DNA Estimation 3.00(184)                     
Operator                                                                          

Instrument Settings
Instrument                     Cary 50                                            
Instrument version no.         3,00                                               
Ordinate Mode                  Abs                                                
Ave Time (sec)                 1,0000                                             
Replicates                     3                                                  
Wavelength 1 (nm)              260,0                                              
Wavelength 2 (nm)              280,0                                              
Background Correction          ON                                                 
Background Wavelength (nm)     320,0                                              
Protein Factor for 260,0 (P1)  757,3000                                           
Protein Factor for 280,0 (P2)  1552,0000                                          
Nucleic Factor for 260,0 (N1)  62,9000                                            
Nucleic Factor for 280,0 (N2)  -36,0000                                           

Ratio = (A[260,0] - Bg[320,0])/(A[280,0] - Bg[320,0])
Protein Conc = ((A[280,0] - Bg[320,0]) * P2) - ((A[260,0] - Bg[320,0]) * P1)
Nucleic Acid Conc = ((A[260,0] - Bg[320,0]) * N1) + ((A[280,0] - Bg[320,0]) * N2)

Analysis
Collection time                2009-08-29 17:57:48                                

    Sample        A[260]     A[280]    Bg[320]     Ratio     Protein   Nucleic Acid     SD       %RSD      Mean    
                                                              µg/ml       µg/ml                                    
___________________________________________________________________________________________________________________
TerminatorB0015     0,0130     0,0077     0,0001     1,7026     1,9917       0,5392                                
                    0,0135     0,0085     0,0007     1,6471     2,3666       0,5251                                
                    0,0137     0,0071     0,0005     1,9912     0,2912       0,5897     0,1847     10,38    1,7803 

RBS-PDC_E+S         0,0026     0,0005    -0,0016     2,0117     0,0600       0,1904                                
                    0,0034     0,0009    -0,0023     1,7729     0,6717       0,2422                                
                    0,0038     0,0008    -0,0024     1,9238     0,3078       0,2751     0,1208      6,35    1,9028 

RBS-Adh2Z_X+P       0,0115     0,0075     0,0044     2,2347    -0,4479       0,3337                                
                    0,0122     0,0085     0,0040     1,8079     0,8228       0,3497                                
                    0,0126     0,0070     0,0045     3,1970    -2,2013       0,4182     0,7115     29,48    2,4132 

RBS-PirB_E+S        0,0131     0,0111     0,0060     1,3904     2,5307       0,2609                                
                    0,0113     0,0052     0,0027     3,4173    -2,6148       0,4517                                
                    0,0096     0,0058     0,0025     2,1635    -0,2819       0,3265     1,0229     44,02    2,3237 

PpetE_E+S           0,0128     0,0066     0,0032     2,8516    -2,0438       0,4823                                
                    0,0133     0,0079     0,0029     2,0977    -0,1816       0,4758                                
                    0,0130     0,0083     0,0035     1,9819     0,2461       0,4269     0,4722     20,44    2,3104 

RBS_X+P             0,0095     0,0061     0,0020     1,8300     0,6755       0,3216                                
                    0,0103     0,0059     0,0021     2,1869    -0,3912       0,3814                                
                    0,0105     0,0055     0,0021     2,4578    -1,0580       0,4057     0,3149     14,59    2,1582 

pSP1AC3_E+P         0,0224     0,0167     0,0094     1,7831     1,4694       0,5549                                
                    0,0205     0,0170     0,0134     1,9943     0,1497       0,3210                                
                    0,0260     0,0206     0,0131     1,7293     1,8137       0,5446     0,1401      7,63    1,8356