RNA - DNA Report

Report time                    2009-09-01 16:46:12                                
Batch name                                                                        
Application                    RNA - DNA Estimation 3.00(184)                     
Operator                                                                          

Instrument Settings
Instrument                     Cary 50                                            
Instrument version no.         3,00                                               
Ordinate Mode                  Abs                                                
Ave Time (sec)                 1,0000                                             
Replicates                     3                                                  
Wavelength 1 (nm)              260,0                                              
Wavelength 2 (nm)              280,0                                              
Background Correction          ON                                                 
Background Wavelength (nm)     320,0                                              
Protein Factor for 260,0 (P1)  757,3000                                           
Protein Factor for 280,0 (P2)  1552,0000                                          
Nucleic Factor for 260,0 (N1)  62,9000                                            
Nucleic Factor for 280,0 (N2)  -36,0000                                           

Ratio = (A[260,0] - Bg[320,0])/(A[280,0] - Bg[320,0])
Protein Conc = ((A[280,0] - Bg[320,0]) * P2) - ((A[260,0] - Bg[320,0]) * P1)
Nucleic Acid Conc = ((A[260,0] - Bg[320,0]) * N1) + ((A[280,0] - Bg[320,0]) * N2)

Analysis
Collection time                2009-09-01 16:46:12                                

    Sample        A[260]     A[280]    Bg[320]     Ratio     Protein   Nucleic Acid     SD       %RSD      Mean    
                                                              µg/ml       µg/ml                                    
___________________________________________________________________________________________________________________
RBS-PirB-T          0,0249     0,0137    -0,0016     1,7282     3,7280       1,1141                                
                    0,0249     0,0128    -0,0016     1,8403     2,2856       1,1509                                
                    0,0248     0,0126    -0,0016     1,8530     2,1165       1,1464     0,0687      3,80    1,8071 

pPetE-RBS           0,0435     0,0226    -0,0029     1,8223     4,3866       2,0054                                
                    0,0437     0,0214    -0,0032     1,9044     2,7051       2,0638                                
                    0,0436     0,0217    -0,0031     1,8832     3,1197       2,0446     0,0426      2,28    1,8700 

RBS B0034           0,0260     0,0151     0,0004     1,7424     3,4166       1,0815                                
                    0,0261     0,0142     0,0001     1,8391     2,2466       1,1242                                
                    0,0260     0,0138     0,0001     1,8944     1,6000       1,1337     0,0769      4,22    1,8253 

F1610               0,0161     0,0073    -0,0026     1,8940     1,1635       0,8217                                
                    0,0160     0,0070    -0,0026     1,9383     0,8070       0,8241                                
                    0,0162     0,0070    -0,0027     1,9414     0,7984       0,8406     0,0265      1,38    1,9245 

R0011               0,0078     0,0027    -0,0018     2,1120    -0,2156       0,4401                                
                    0,0084     0,0027    -0,0018     2,2800    -0,7747       0,4765                                
                    0,0081     0,0033    -0,0018     1,9615     0,3346       0,4390     0,1593      7,52    2,1178 

F2622               0,0177     0,0078    -0,0028     1,9251     1,0021       0,9062                                
                    0,0175     0,0077    -0,0024     1,9698     0,6098       0,8897                                
                    0,0176     0,0080    -0,0026     1,9041     1,1676       0,8892     0,0336      1,74    1,9330