Imperial College London/Notebook/17 September 2009

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==Testing==

===OD calibration 17th Sep===

====Protocol considerations====

*Cells were grown overnight in 10ml of M9 media
*The cells were allowed to grow until a high OD (very cloudy) and retrieved in the early afternoon
*The OD was measured using the multiplate reader.
*As the OD was only around 1.5, the cells were centrifuged and resuspended in less media to form an OD of 3
*OD of around 0.5 to 3.0 was made up, and subsequently diluted up to 10<sup>-6</sup> to 10<sup>-8</sup>
*Plated on strep plates and dried until the end of the day
*Placed into 37 degrees incubator (non-shaking) overnight

====Results====

The plates had too many colonies to count. 
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====Conclusions====

* The plates were all too confluent, even at 10<sup>-8</sup> dilution.
* Unable to count colonies and draw any colonies
* Need to repeat experiment


====Suggestions/Improvements====

*Was not done on ice, so cell growth still occurred during dilutions
*Too many colonies to count when plated 100uL
**Use ice
**Increase dilutions


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