Team:Kyoto/Cells in Cells/Notebook/week1

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===Project abstract===
abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract 
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  <li><a id="calendar_week1" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week1" title="1st week">1st week</a></li>
  <li><a id="calendar_week2" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week2" title="2nd week">2nd week</a></li>
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===Contents===

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===Monday===
====To Do on 24 July====
*Miniprep (3-O-2, 3-A-4)
*Restriction Enzyme Digestion
*Electrophoresis

====Results====
=====Miniprep=====
*3-O-2, 3-A-4 was minipreped.

=====Digestion=====
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.

{| border="1" style="text-align:center;width:50%"
|+Sample list
!NO.!!Sample Name!!Enzyme
|-align="center"
|1||3-O-2||Eco R1, PST1
|-align="center"
|2||3-A-4||Eco R1, PST1
|-align="center"
|3||3-O-2||Eco R1
|-align="center"
|4||3-A-4||Eco R1
|}

=====Electrophoresis=====
[[Image:Kyoto_e_090724_1.png|250px]]
* Very clear bands were found. (See the gel image in right!)
* The bands are listed below.

{| border="1" style="text-align:center;width:50%"
|+Parts and Plasmid length
!!!3-O-2!!3-A-4
|-
|Parts length / bp||2837||487
|-
|Plasmid length / bp||3266||3266
|}
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===Tuesday===
====To Do on 24 July====
*Miniprep (3-O-2, 3-A-4)
*Restriction Enzyme Digestion
*Electrophoresis

====Results====
=====Miniprep=====
*3-O-2, 3-A-4 was minipreped.

=====Digestion=====
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.

{| border="1" style="text-align:center;width:50%"
|+Sample list
!NO.!!Sample Name!!Enzyme
|-align="center"
|1||3-O-2||Eco R1, PST1
|-align="center"
|2||3-A-4||Eco R1, PST1
|-align="center"
|3||3-O-2||Eco R1
|-align="center"
|4||3-A-4||Eco R1
|}

=====Electrophoresis=====
[[Image:Kyoto_e_090724_1.png|250px]]
* Very clear bands were found. (See the gel image in right!)
* The bands are listed below.

{| border="1" style="text-align:center;width:50%"
|+Parts and Plasmid length
!!!3-O-2!!3-A-4
|-
|Parts length / bp||2837||487
|-
|Plasmid length / bp||3266||3266
|}
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</div><!-- /.minipad_center -->
<div class="minipad_bottom"></div>
<div class="minipad_top"></div>
<div class="minipad_center">
</html>
===Wednesday===
====To Do on 24 July====
*Miniprep (3-O-2, 3-A-4)
*Restriction Enzyme Digestion
*Electrophoresis

====Results====
=====Miniprep=====
*3-O-2, 3-A-4 was minipreped.

=====Digestion=====
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.

{| border="1" style="text-align:center;width:50%"
|+Sample list
!NO.!!Sample Name!!Enzyme
|-align="center"
|1||3-O-2||Eco R1, PST1
|-align="center"
|2||3-A-4||Eco R1, PST1
|-align="center"
|3||3-O-2||Eco R1
|-align="center"
|4||3-A-4||Eco R1
|}

=====Electrophoresis=====
[[Image:Kyoto_e_090724_1.png|250px]]
* Very clear bands were found. (See the gel image in right!)
* The bands are listed below.

{| border="1" style="text-align:center;width:50%"
|+Parts and Plasmid length
!!!3-O-2!!3-A-4
|-
|Parts length / bp||2837||487
|-
|Plasmid length / bp||3266||3266
|}
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</div><!-- /.minipad_center -->
<div class="minipad_bottom"></div>
<div class="minipad_top"></div>
<div class="minipad_center">
</html>
===Thursday===
====To Do on 24 July====
*Miniprep (3-O-2, 3-A-4)
*Restriction Enzyme Digestion
*Electrophoresis

====Results====
=====Miniprep=====
*3-O-2, 3-A-4 was minipreped.

=====Digestion=====
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.

{| border="1" style="text-align:center;width:50%"
|+Sample list
!NO.!!Sample Name!!Enzyme
|-align="center"
|1||3-O-2||Eco R1, PST1
|-align="center"
|2||3-A-4||Eco R1, PST1
|-align="center"
|3||3-O-2||Eco R1
|-align="center"
|4||3-A-4||Eco R1
|}

=====Electrophoresis=====
[[Image:Kyoto_e_090724_1.png|250px]]
* Very clear bands were found. (See the gel image in right!)
* The bands are listed below.

{| border="1" style="text-align:center;width:50%"
|+Parts and Plasmid length
!!!3-O-2!!3-A-4
|-
|Parts length / bp||2837||487
|-
|Plasmid length / bp||3266||3266
|}
<html>
</div><!-- /.minipad_center -->
<div class="minipad_bottom"></div>
<div class="minipad_top"></div>
<div class="minipad_center">
</html>
===Friday===
====To Do on 24 July====
*Miniprep (3-O-2, 3-A-4)
*Restriction Enzyme Digestion
*Electrophoresis

====Results====
=====Miniprep=====
*3-O-2, 3-A-4 was minipreped.

=====Digestion=====
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.

{| border="1" style="text-align:center;width:50%"
|+Sample list
!NO.!!Sample Name!!Enzyme
|-align="center"
|1||3-O-2||Eco R1, PST1
|-align="center"
|2||3-A-4||Eco R1, PST1
|-align="center"
|3||3-O-2||Eco R1
|-align="center"
|4||3-A-4||Eco R1
|}

=====Electrophoresis=====
[[Image:Kyoto_e_090724_1.png|250px]]
* Very clear bands were found. (See the gel image in right!)
* The bands are listed below.

{| border="1" style="text-align:center;width:50%"
|+Parts and Plasmid length
!!!3-O-2!!3-A-4
|-
|Parts length / bp||2837||487
|-
|Plasmid length / bp||3266||3266
|}
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