Team:Kyoto/CiC/Notebook/1019-1023

{{:Team:Kyoto/Header2}}
<html>
<script type="text/javascript">
<!--
  $(function(){
    initialize("CiC"); // <== initialize("GSDD" or "CiC"); 1)
  });
// -->
</script>
<style type="text/css">
/* ul#navi_GSDD or ul#navi_CiC */
/* li.nav_i, li.nav_me, li.nav_g, li.nav_r, li.nav_p, li.nav_mo, li.nav_n  */

#navigation ul#navi_CiC li ul li.nav_n a{
  background-position:left bottom !important;
  color:black;
}
</style></html>
<div id="topicpath">
#[[Team:Kyoto|Home]]
#[[Team:Kyoto/CiC|CiC]]
#Notebook
</div><!-- /#topicpath -->
{{:Team:Kyoto/Leftnavi}}

<!-- ** Edit below ** -->
<html>
<div id="rightcontents">
</html>
{{:Team:Kyoto/CiC/Notebook_tpl}}
==1019-1025 :Obserbation  ==

{{:Team:Kyoto/Pad_start_dates}}
===Monday, 19 October===
====To Do====
* subgoal A --- Confirmation of function of TAT-liposome
**observation by microscope
* Subgoal B --- Liposomes with Mitochondrial Translocases
**Mitochondoria isolation
**expressing sig-GFP in vitro with mitochondoria
* Subgoal C --- sig-EGFP expression in HeLa
**transfomation

====Results====
=====subgoal A --- Confirmation of function of TAT-liposome=====
Liposome was taken by HeLa cells.See the [[Team:Kyoto/CiC/Results|results]]. 

=====Subgoal C --- sig-EGFP expression in HeLa=====
sig-EGFP　was made a localization in mitochondoria.
See the [[Team:Kyoto/CiC/Results|results]]. 

{{:Team:Kyoto/Pad_between_dates}}

===Tuesday, 20 October===
====To Do====
* Subgoal B --- Liposomes with Mitochondrial Translocases
**observation with microscope
====Results====
See the [[Team:Kyoto/CiC/Results|results]]. 

{{:Team:Kyoto/Pad_between_dates}}

===Wednesday, 21 October===
====To Do====
* subgoal A --- Confirmation of function of TAT-liposome
**making liposome by natural swelling method and extruder
**add liposome to HeLa cells
====Results====
According to the expectation,HIV-TAT-(LALAAAA)3-generator may work.
Liposome is taken by HeLa Cells.
See the [[Team:Kyoto/CiC/Results|results]]. 

{{:Team:Kyoto/Pad_endof_dates}}
<html></div><!-- /#rightcontents --></html>