Team:NYMU-Taipei/Sequences/Virus Proteins

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== Sequences and Primers ==
=== H1N1 NA ===
{{:Team:NYMU-Taipei/Seq|ATGAACCCCAACCAGAAGATCATCACCATCGGCAGCGTGTGCATGACCATCGGCATGGCCAACCTGATC{{:Team:NYMU-Taipei/BBCut|CTGCAG}}ATCGG
CAACATCATCAGCATCTGGATCAGCCACAGCATCCAGCTGGGCAACCAGAACCAGATCGAGACCTGCAACCAGAGCGTGA
TCACCTACGAGAACAACACCTGGGTGAACCAGACCTACGTGAACATCAGCAACACCAACTTCGCCGCCGGCCAGAGCGTG
GTGAGCGTGAAGCTGGCCGGCAACAGCAGCCTGTGCCCCGTGAGCGGCTGGGCCATCTACAGCAAGGACAACAGCGTGAG
GATCGGCAGCAAGGGCGACGTGTTCGTGATCAGGGAGCCCTTCATCAG{{:Team:NYMU-Taipei/BBCut|CTGCAG}}CCCCCTGGAGTGCAGGACCTTCTTCC
TGACCCAGGGCGCCCTGCTGAACGACAAGCACAGCAACGGCACCATCAAGGACAGGAGCCCCTACAGGACCCTGATGAGC
TGCCCCATCGGCGAGGTGCCCAGCCCCTACAACAGCAGGTTCGAGAGCGTGGCCTGGAGCGCCAGCGCCTGCCACGACGG
CATCAACTGGCTGACCATCGGCATCAGCGGCCCCGACAACGGCGCCGTGGCCGTGCTGAAGTACAACGGCATCATCACCG
ACACCATCAAGAGCTGGAGGAACAACATCCTGAGGACCCAGGAGAGCGAGTGCGCCTGCGTGAACGGCAGCTGCTTCACC
GTGATGACCGACGGCCCCAGCAACGGCCAGGCCAGCTACAAGATCTTCAGGATCGAGAAGGGCAAGATCGTGAAGAGCGT
GGAGATGAACGCCCCCAACTACCACTACGAGGAGTGCAGCTGCTACCCCGACAGCAGCGAGATCACCTGCGTGTGCAGGG
ACAACTGGCACGGCAGCAACAGGCCCTGGGTGAGCTTCAACCAGAACCTGGAGTACCAGATCGGCTACAT{{:Team:NYMU-Taipei/BBCut|CTGCAG}}CGGC
ATCTTCGGCGACAACCCCAGGCCCAACGACAAGACCGGCAGCTGCGGCCCCGTGAGCAGCAACGGCGCCAACGGCGTGAA
GGGCTTCAGCTTCAAGTACGGCAACGGCGTGTGGATCGGCAGGACCAAGAGCATCAGCAGCAGGAACGGCTTCGAGATGA
TCTGGGACCCCAACGGCTGGACCGGCACCGACAACAACTTCAGCATCAAGCAGGACATCGTGGGCATCAACGAGTGGAGC
GGCTACAGCGGCAGCTTCGTGCAGCACCCCGAGCTGACCGGCCTGGACTGCATCAGGCCCTGCTTCTGGGTGGAGCTGAT
CAGGGGCAGGCCCAAGGAGAACACCATCTGGACCAGCGGCAGCAGCATCAGCTTCTGCGGCGTGAACAGCGACACCGTGG
GCTGGAGCTGGCCCGACGGCGCCGAGCTGCCCTTCACCATCGACAAGTGACTCGAGTAA|H1N1 NA orignal|1419}}

{{:Team:NYMU-Taipei/Seq|{{:Team:NYMU-Taipei/Primer|ATGAACCCCAACCAGAAGA|f primer}}TCATCACCATCGGCAGCGTGTGCATGACCATCGGCATGGCCAAC{{Team:NYMU-Taipei/Primer|CTGATC{{:Team:NYMU-Taipei/BBCut|CTaCAG|mut}}ATCGG
CAA|primer2}}CATCATCAGCATCTGGATCAGCCACAGCATCCAGCTGGGCAACCAGAACCAGATCGAGACCTGCAACCAGAGCGTGA
TCACCTACGAGAACAACACCTGGGTGAACCAGACCTACGTGAACATCAGCAACACCAACTTCGCCGCCGGCCAGAGCGTG
GTGAGCGTGAAGCTGGCCGGCAACAGCAGCCTGTGCCCCGTGAGCGGCTGGGCCATCTACAGCAAGGACAACAGCGTGAG
GATCGGCAGCAAGGGCGACGTGTTCGTGATCAGGGAGCC{{Team:NYMU-Taipei/Primer|CTTCATCAG{{:Team:NYMU-Taipei/BBCut|CTGtAG|mut}}CCCC|primer2}}CTGGAGTGCAGGACCTTCTTCC
TGACCCAGGGCGCCCTGCTGAACGACAAGCACAGCAACGGCACCATCAAGGACAGGAGCCCCTACAGGACCCTGATGAGC
TGCCCCATCGGCGAGGTGCCCAGCCCCTACAACAGCAGGTTCGAGAGCGTGGCCTGGAGCGCCAGCGCCTGCCACGACGG
CATCAACTGGCTGACCATCGGCATCAGCGGCCCCGACAACGGCGCCGTGGCCGTGCTGAAGTACAACGGCATCATCACCG
ACACCATCAAGAGCTGGAGGAACAACATCCTGAGGACCCAGGAGAGCGAGTGCGCCTGCGTGAACGGCAGCTGCTTCACC
GTGATGACCGACGGCCCCAGCAACGGCCAGGCCAGCTACAAGATCTTCAGGATCGAGAAGGGCAAGATCGTGAAGAGCGT
GGAGATGAACGCCCCCAACTACCACTACGAGGAGTGCAGCTGCTACCCCGACAGCAGCGAGATCACCTGCGTGTGCAGGG
ACAACTGGCACGGCAGCAACAGGCCCTGGGTGAGCTTCAACCAGAACCTGGAGTACCAGATCGGC{{Team:NYMU-Taipei/Primer|TACAT{{:Team:NYMU-Taipei/BBCut|CTGtAG|mut}}CGGC
ATCTT|primer2}}CGGCGACAACCCCAGGCCCAACGACAAGACCGGCAGCTGCGGCCCCGTGAGCAGCAACGGCGCCAACGGCGTGAA
GGGCTTCAGCTTCAAGTACGGCAACGGCGTGTGGATCGGCAGGACCAAGAGCATCAGCAGCAGGAACGGCTTCGAGATGA
TCTGGGACCCCAACGGCTGGACCGGCACCGACAACAACTTCAGCATCAAGCAGGACATCGTGGGCATCAACGAGTGGAGC
GGCTACAGCGGCAGCTTCGTGCAGCACCCCGAGCTGACCGGCCTGGACTGCATCAGGCCCTGCTTCTGGGTGGAGCTGAT
CAGGGGCAGGCCCAAGGAGAACACCATCTGGACCAGCGGCAGCAGCATCAGCTTCTGCGGCGTGAACAGCGACACCGTGG
GCTGGAGCTGGCCCGACGGCGCCGAGCTGCCCTTCAC{{:Team:NYMU-Taipei/Primer|CATCGACAAGTGACTCGAGTAA|r primer}}|H1N1 NA mutant|1419}}

outer primer
 gaattcgcggccgcttctag ATGAACCCCAACCAGAAGA 55deg, GC:47%, 20+19=39bp
 ctgcagcggccgctactagta TTACTCGAGTCACTTGTCGATG 55deg, GC:45%, 21+22=43bp
inner 1: PstI@pos70, 55deg, GC:50%, 20bp
 CTGATCCTaCAGATCGGCAA
 TTGCCGATCTGtAGGATCAG
inner 2: PstI@pos369, 56deg, GC:58%, 19bp
 CTTCATCAGCTGtAGCCCC
 GGGGCTaCAGCTGATGAAG
inner 3: PstI@pos951, 55deg, GC:45%, 20bp
 TACATCTGtAGCGGCATCTT
 AAGATGCCGCTaCAGATGTA

=== H1N1 HA ===
{{:Team:NYMU-Taipei/Seq|{{:Team:NYMU-Taipei/Primer|ATGAAGGCCATCCTGGTG|f primer}}GTGCTGCTGTACACCTTCGCCACCGCCAACGCCGACACCCTGTGCATCGGCTACCACGCCA
ACAACAGCACCGACACCGTGGACACCGTGCTGGAGAAGAACGTGACCGTGACCCACAGCGTGAACCTGCTGGAGGACAA
GCACAACGGCAAGCTGTGCAAGCTGAGGGGCGTGGCCCCCCTGCACCTGGGCAAGTGCAACATCGCCGGCTGGATCCTG
GGCAACCCCGAGTGCGAGAGCCTGAGCACCGCCAGCAGCTGGAGCTACATCGTGGAGACCCCCAGCAGCGACAACGGCA
CCTGCTACCCCGGCGACTTCATCGACTACGAGGAGCTGAGGGAGCAGCTGAGCAGCGTGAGCAGCTTCGAGAGGTTCGA
GATCTTCCCCAAGACCAGCAGCTGGCCCAACCACGACAGCAACAAGGGCGTGACCGCCGCCTGCCCCCACGCCGGCGCC
AAGAGCTTCTACAAGAACCTGATCTGGCTGGTGAAGAAGGGCAACAGCTACCCCAAGCTGAGCAAGAGCTACATCAACG
ACAAGGGCAAGGAGGTGCTGGTGCTGTGGGGCATCCACCACCCCAGCACCAGCGCCGACCAGCAGAGCCTGTACCAGAA
CGCCGACACCTACGTGTTCGTGGGCAGCAGCAGGTACAGCAAGAAGTTCAAGCCCGAGATCGCCATCAGGCCCAAGGTG
AGGGACCAGGAGGGCAGGATGAACTACTACTGGACCCTGGTGGAGCCCGGCGACAAGATCACCTTCGAGGCCACCGGCA
ACCTGGTGGTGCCCAGGTACGCCTTCGCCATGGAGAGGAACGCCGGCAGCGGCATCATCATCAGCGACACCCCCGTGCA
CGACTGCAACACCACCTGCCAGACCCCCAAGGGCGCCATCAACACCAGCCTGCCCTTCCAGAACATCCACCCCATCACC
ATCGGCAAGTGCCCCAAGTACGTGAAGAGCACCAAGCTGAGGCTGGCCACCGGCCTGAGGAACATCCCCCAGATCGAGA
CCAGGGGCCTGTTCGGCGCCATCGCCGGCTTCATCGAGGGCGGCTGGACCGGCATGGTGGACGGCTGGTACGGCTACCA
CCACCAGAACGAGCAGGGCAGCGGCTACGCCGCCGACCTGAAGAGCACCCAGAACGCCATCGACGAGATCACCAACAAG
GTGAACAGCGTGATCGAGAAGATGAACACCCAGTTCACCGCCGTGGGCAAGGAGTTCAACCACCTGGAGAAGAGGATCG
AGAACCTGAACAAGAAGGTGGACGACGGCTTCCTGGACATCTGGACCTACAACGCCGAGCTGCTGGTGCTGCTGGAGAA
CGAGAGGACCCTGGACTACCACGACAGCAACGTGAAGAACCTGTACGAGAAGGTGAGGAGCCAGCTGAAGAACAACGCC
AAGGAGATCGGCAACGGCTGCTTCGAGTTCTACCACAAGTGCGACAACACCTGCATGGAGAGCGTGAAGAACGGCACCT
ACGACTACCCCAAGTACAGCGAGGAGGCCAAGCTGAACAGGGAGGAGATCGACGGCGTGAAGCTGGAGAGCACCAGGAT
CTACCAGATCCTGGCCATCTACAGCACCGTGGCCAGCAGCCTGGTGCTGGTGGTGAGCCTGGGCGCCATCAGCTTCTGG
ATGTGCAGCAACGGCAG{{:Team:NYMU-Taipei/Primer|CCTGCAGTGCAGGATCTGCATCTGA|r primer}}|H1N1 HA|1701}}
outer primer:
 gaattcgcggccgcttctag ATGAAGGCCATCCTGGTG 55deg, GC:56%, 20+18=38bp
 ctgcagcggccgctactagta TCAGATGCAGATCCTGCAtTGCAGG 63deg, GC:52%, 21+25=46bp

=== H5N1 NA ===
{{:Team:NYMU-Taipei/Seq|ATGAATCCAAATCAGAAGATAATAACCATCGGATCAATCTGTATGGTAACTGGAATAGTTAGCTTAATGTTACAAATTG
GGAACATGATCTCAATATGGGTCAGTCATTCAATTCACACAGGGAATCAACACCAATCTGAACCAATCAGCAATACTAA
TTTTCTTACTGAGAAAGCTGTGGCTTCAGTAAAATTAGCGGGCAATTCATCTCTTTGCCCCATTAACGGATGGGCTGTA
TACAGTAAGGACAACAGTATAAGGATCGGTTCCAAGGGGGATGTGTTTGTTATAAGAGAGCCGTTCATCTCATGCTCCC
ACTTGGAATGCAGAACTTTCTTTTTGACTCAGGGAGCCTTGCTGAATGACAAGCACTCCAATGGGACTGTCAAAGACAG
AAGCCCTCACAGAACATTAATGAGTTGTCCTGTGGGTGAGGCTCCCTCCCCATATAACTCAAGGTTTGAGTCTGTTGCT
TGGTCAGCAAGTGCTTGCCATGATGGCACCAGTTGGTTGACGATTGGAATTTCTGGCCCAGACAATGGGGCTGTGGCTG
TATTGAAATACAATGGCATAATAACAGACACTATCAAGAGTTGGAGGAACAACATACTGAGAACTCAAGAGTCTGAATG
TGCATGTGTAAATGGCTCTTGCTTTACTGTAATGACTGACGGACCAAGTAATGGTCAGGCATCACATAAGATCTTCAAA
ATGGAAAAAGGGAAAGTGGTTAAATCAGTCGAATTGGATGCTCCTAATTATCACTATGAGGAATGCTCCTGTTATCCTA
ATGCCGGAGAAATCACATGTGTGTGCAGGGATAATTGGCATGGCTCAAATCGGCCATGGGTATCTTTCAATCAAAATTT
GGAGTATCAAATAGGATATATATGCAGTGGAGTTTTCGGAGACAATCCACGCCCCAATGATGGAACAGGTAGTTGTGGT
CCGGTGTCCTCTAACGGGGCATATGGGGTAAAAGGGTTTTCATTTAAATACGGCAATGGTGTCTGGATCGGGAGAACCA
AAAGCACTAATTCCAGGAGCGGCTTTGAAATGATTTGGGATCCAAATGGGTGGACTGAAACGGACAGTAGCTTTTCAGT
GAAACAAGATATCGTAGCAATAACTGATTGGTCAGGATATAGCGGGAGTTTTGTCCAGCATCCAGAACTGACAGGACTA
GATTGCATAAGACCTTGTTTCTGGGTTGAGTTGATCAGAGGGCGGCCCAAAGAGAGCACAATTTGG{{Team:NYMU-Taipei/BBCut|ACTAGT}}GGGAGCA
GCATATCTTTTTGTGGTGTAAATAGTGACACTGTGGGTTGGTCTTGGCCAGACGGTGCTGAGTTGCCATTCACCATTGA
CAAGTAG|H5N1 NA original|1350}}

{{:Team:NYMU-Taipei/Seq|{{Team:NYMU-Taipei/Primer|ATGAATCCAAATCAGAAGATAATAACCA|f primer}}TCGGATCAATCTGTATGGTAACTGGAATAGTTAGCTTAATGTTACAAATTG
GGAACATGATCTCAATATGGGTCAGTCATTCAATTCACACAGGGAATCAACACCAATCTGAACCAATCAGCAATACTAA
TTTTCTTACTGAGAAAGCTGTGGCTTCAGTAAAATTAGCGGGCAATTCATCTCTTTGCCCCATTAACGGATGGGCTGTA
TACAGTAAGGACAACAGTATAAGGATCGGTTCCAAGGGGGATGTGTTTGTTATAAGAGAGCCGTTCATCTCATGCTCCC
ACTTGGAATGCAGAACTTTCTTTTTGACTCAGGGAGCCTTGCTGAATGACAAGCACTCCAATGGGACTGTCAAAGACAG
AAGCCCTCACAGAACATTAATGAGTTGTCCTGTGGGTGAGGCTCCCTCCCCATATAACTCAAGGTTTGAGTCTGTTGCT
TGGTCAGCAAGTGCTTGCCATGATGGCACCAGTTGGTTGACGATTGGAATTTCTGGCCCAGACAATGGGGCTGTGGCTG
TATTGAAATACAATGGCATAATAACAGACACTATCAAGAGTTGGAGGAACAACATACTGAGAACTCAAGAGTCTGAATG
TGCATGTGTAAATGGCTCTTGCTTTACTGTAATGACTGACGGACCAAGTAATGGTCAGGCATCACATAAGATCTTCAAA
ATGGAAAAAGGGAAAGTGGTTAAATCAGTCGAATTGGATGCTCCTAATTATCACTATGAGGAATGCTCCTGTTATCCTA
ATGCCGGAGAAATCACATGTGTGTGCAGGGATAATTGGCATGGCTCAAATCGGCCATGGGTATCTTTCAATCAAAATTT
GGAGTATCAAATAGGATATATATGCAGTGGAGTTTTCGGAGACAATCCACGCCCCAATGATGGAACAGGTAGTTGTGGT
CCGGTGTCCTCTAACGGGGCATATGGGGTAAAAGGGTTTTCATTTAAATACGGCAATGGTGTCTGGATCGGGAGAACCA
AAAGCACTAATTCCAGGAGCGGCTTTGAAATGATTTGGGATCCAAATGGGTGGACTGAAACGGACAGTAGCTTTTCAGT
GAAACAAGATATCGTAGCAATAACTGATTGGTCAGGATATAGCGGGAGTTTTGTCCAGCATCCAGAACTGACAGGACTA
GATTGCATAAGACCTTGTTTCTGGGTTGAGTTGATCAGAGGGCGGCCCAAAGAGAGCACA{{Team:NYMU-Taipei/Primer|ATTTGG{{Team:NYMU-Taipei/BBCut|ACcAGT|mut}}GGGAGC|primer2}}A
GCATATCTTTTTGTGGTGTAAATAGTGACACTGTGGGTTGGTCTTGGCCAGACGGTGCTGAGTT{{Team:NYMU-Taipei/Primer|GCCATTCACCATTGA
CAAGTAG|r primer}}|H5N1 NA mutant|1350}}

outer primer:
 gaattcgcggccgcttctag ATGAATCCAAATCAGAAGATAATAACCA 55deg, GC:29%, 20+28=48bp
 ctgcagcggccgctactagta CTACTTGTCAATGGTGAATGGC 55deg, GC:45%, 21+22=43bp
inner primer: SpeI@pos1252, 56deg, GC:56%, 18bp
 ATTTGGACcAGTGGGAGC
 GCTCCCACTgGTCCAAAT

=== H5N1 HA ===
{{:Team:NYMU-Taipei/Seq|ATGGAGAAAATAGTGCTTCTTTTTGCAATAGTCAGTCTTGTTAAAAGTGATCAGATTTGCATTGGTTACCATGCAAACA
ACTCGACAGAGCAGGTTGACACAATAATGGAAAAGAACGTTACTGTTACACATGCCCAAGACATACTGGAAAAGAAACA
CAACGGGAAGCTCTGCGATCTAGATGGAGTGAAGCCTCTAATTTTGAGAGATTGTAGCGTAGCTGGATGGCTCCTCGGA
AACCCAATGTGTGAC{{:Team:NYMU-Taipei/BBCut|GAATTC}}ATCAATGTGCCGGAATGGTCTTACATAGTGGAGAAGGCCAATCCAGTCAATGACCTCT
GTTACCCAGGGGATTTCAATGACTATGAAGAATTGAAACACCTATTGAGCAGAATAAACCATTTTGAGAAAATTCAGAT
CATCCCCAAAAGTTCTTGGTCCAGTCATGAAGCCTCATTAGGGGTGAGCTCAGCATGTCCATACCAGGGAAAGTCCTCC
TTTTTCAGAAATGTGGTATGGCTTATCAAAAAGAACAGTACATACCCAACAATAAAGAGGAGCTACAATAATACCAACC
AAGAAGATCTTTTGGTACTGTGGGGGATTCACCATCCTAATGATGCGGCAGAGCAGACAAAGCTCTATCAAAACCCAAC
CACCTATATTTCCGTTGGGACATCAACACTAAACCAGAGATTGGTACCAAGAATAGCTACTAGATCCAAAGTAAACGGG
CAAAGTGGAAGGATGGAGTTCTTCTGGACAATTTTAAAGCCGAATGATGCAATCAACTTCGAGAGTAATGGAAATTTCA
TTGCTCCAGAATATGCATACAAAATTGTCAAGAAAGGGGACTCAACAATTATGAAAAGTGAATTGGAATATGGTAACTG
CAACACCAAGTGTCAAACTCCAATGGGGGCGATAAACTCTAGCATGCCATTCCACAATATACACCCTCTCACCATTGGG
GAATGCCCCAAATATGTGAAATCAAACAGATTAGTCCTTGCGACTGGGCTCAGAAATAGCCCTCAAAGAGAGAGAAGAA
GAAAAAAGAGAGGATTATTTGGAGCTATAGCAGGTTTTATAGAGGGAGGATGGCAGGGAATGGTAGATGGTTGGTATGG
GTACCACCATAGCAATGAGCAGGGGAGTGGGTACG{{:Team:NYMU-Taipei/BBCut|CTGCAG}}ACAAAGAATCCACTCAAAAGGCAATAGATGGAGTCACC
AATAAGGTCAACTCGATCATTGACAAAATGAACACTCAGTTTGAGGCCGTTGGAAGGGAATTTAACAACTTAGAAAGGA
GAATAGAGAATTTAAACAAGAAGATGGAAGACGGGTTCCTAGATGTCTGGACTTATAATGCTGAACTTCTGGTTCTCAT
GGAAAATGAGAGAAC{{:Team:NYMU-Taipei/BBCut|TCTAGA}}CTTTCATGACTCAAATGTCAAGAACCTTTACGACAAGGTCCGACTACAGCTTAGGGAT
AATGCAAAGGAGCTGGGTAACGGTTGTTTCGAGTTCTATCATAAATGTGATAATGAATGTATGGAAAGTGTAAGAAATG
GAACGTATGACTACCCGCAGTATTCAGAAGAAGCGAGACTAAAAAGAGAGGAAATAAGTGGAGTAAAATTGGAATCAAT
AGGAATTTACCAAATACTGTCAATTTATTCTACAGTGGCGAGTTCCCTAGCACTGGCAATCATGGTAGCTGGTCTATCC
TTATGGATGTGCTCCAATGGGTCGTTACAATGCAGAATTTGCATTTAA|H5N1 HA original|1701}}

{{:Team:NYMU-Taipei/Seq|{{Team:NYMU-Taipei/Primer|ATGGAGAAAATAGTGCTTCTTTTTG|f primer}}CAATAGTCAGTCTTGTTAAAAGTGATCAGATTTGCATTGGTTACCATGCAAACA
ACTCGACAGAGCAGGTTGACACAATAATGGAAAAGAACGTTACTGTTACACATGCCCAAGACATACTGGAAAAGAAACA
CAACGGGAAGCTCTGCGATCTAGATGGAGTGAAGCCTCTAATTTTGAGAGATTGTAGCGTAGCTGGATGGCTCCTCGGA
AACCCAA{{Team:NYMU-Taipei/Primer|TGTGTGAC{{:Team:NYMU-Taipei/BBCut|GAgTTC|mut}}ATCAATG|primer2}}TGCCGGAATGGTCTTACATAGTGGAGAAGGCCAATCCAGTCAATGACCTCT
GTTACCCAGGGGATTTCAATGACTATGAAGAATTGAAACACCTATTGAGCAGAATAAACCATTTTGAGAAAATTCAGAT
CATCCCCAAAAGTTCTTGGTCCAGTCATGAAGCCTCATTAGGGGTGAGCTCAGCATGTCCATACCAGGGAAAGTCCTCC
TTTTTCAGAAATGTGGTATGGCTTATCAAAAAGAACAGTACATACCCAACAATAAAGAGGAGCTACAATAATACCAACC
AAGAAGATCTTTTGGTACTGTGGGGGATTCACCATCCTAATGATGCGGCAGAGCAGACAAAGCTCTATCAAAACCCAAC
CACCTATATTTCCGTTGGGACATCAACACTAAACCAGAGATTGGTACCAAGAATAGCTACTAGATCCAAAGTAAACGGG
CAAAGTGGAAGGATGGAGTTCTTCTGGACAATTTTAAAGCCGAATGATGCAATCAACTTCGAGAGTAATGGAAATTTCA
TTGCTCCAGAATATGCATACAAAATTGTCAAGAAAGGGGACTCAACAATTATGAAAAGTGAATTGGAATATGGTAACTG
CAACACCAAGTGTCAAACTCCAATGGGGGCGATAAACTCTAGCATGCCATTCCACAATATACACCCTCTCACCATTGGG
GAATGCCCCAAATATGTGAAATCAAACAGATTAGTCCTTGCGACTGGGCTCAGAAATAGCCCTCAAAGAGAGAGAAGAA
GAAAAAAGAGAGGATTATTTGGAGCTATAGCAGGTTTTATAGAGGGAGGATGGCAGGGAATGGTAGATGGTTGGTATGG
GTACCACCATAGCAATGAGCAGGGGAGTGGG{{Team:NYMU-Taipei/Primer|TACG{{:Team:NYMU-Taipei/BBCut|CTGCcG|mut}}ACAAAGAA|primer2}}TCCACTCAAAAGGCAATAGATGGAGTCACC
AATAAGGTCAACTCGATCATTGACAAAATGAACACTCAGTTTGAGGCCGTTGGAAGGGAATTTAACAACTTAGAAAGGA
GAATAGAGAATTTAAACAAGAAGATGGAAGACGGGTTCCTAGATGTCTGGACTTATAATGCTGAACTTCTGGTTCTCAT
GGAAAAT{{Team:NYMU-Taipei/Primer|GAGAGAAC{{:Team:NYMU-Taipei/BBCut|TCTgGA|mut}}CTTTCATGA|primer2}}CTCAAATGTCAAGAACCTTTACGACAAGGTCCGACTACAGCTTAGGGAT
AATGCAAAGGAGCTGGGTAACGGTTGTTTCGAGTTCTATCATAAATGTGATAATGAATGTATGGAAAGTGTAAGAAATG
GAACGTATGACTACCCGCAGTATTCAGAAGAAGCGAGACTAAAAAGAGAGGAAATAAGTGGAGTAAAATTGGAATCAAT
AGGAATTTACCAAATACTGTCAATTTATTCTACAGTGGCGAGTTCCCTAGCACTGGCAATCATGGTAGCTGGTCTATCC
TTATGGATGTGCTCCAATGGGT{{Team:NYMU-Taipei/Primer|CGTTACAATGCAGAATTTGCATTTAA|r primer}}|H5N1 HA mutant|1701}}

outer primer:
 gaattcgcggccgcttctag ATGGAGAAAATAGTGCTTCTTTTTG 54deg, GC:32%, 20+25=45bp
 ctgcagcggccgctactagta TTAAATGCAAATTCTGCATTGTAACG 55deg, GC:31%, 21+26=47bp
inner primer1: EcoRI@pos253, 54deg, GC:43%, 21bp
 TGTGTGACGAgTTCATCAATG
 CATTGATGAAcTCGTCACACA
inner primer2: PstI@pos1142, 55deg, GC:50%, 18bp
 TACGCTGCcGACAAAGAA
 TTCTTTGTCgGCAGCGTA
inner primer3: XbaI@pos1359, 55deg, GC:43%, 23bp
 GAGAGAACTCTgGACTTTCATGA
 TCATGAAAGTCcAGAGTTCTCTC

=== HIV-1 gp120 ===
{{:Team:NYMU-Taipei/Seq|ATGAGAGTGACGGGGATCAGGAAGAATTATCGGCACTTATGGAAATGGGGCACCATGCTCCTTGGAATGTTGATGATCT
GTAGTGCTGTAGAAAACTTGTGGGTCACAGTCTATTATGGGGTACCTATATGGAAAGATGCAACCACCACTTTATTTTG
TGCATCAGATGCTAGAGCATATGATACAGAGGTACATAATGTTTGGGCTACACATGCCTGTGTACCCACAGACCCCAAC
CCACAAGAACTGGTTTTGGTAAATGTAACAGAAAATTTTAACATGTGGAAAAATGACATGGTAGATCAGATGCATGAAG
ATGTAATCAGTTTATGGGATCAAAGCCTAAAGCCATGTGTAAAGTTGACCCCACTCTGTGTCACTTTAAACTGTACAAA
TGCTACCCGTAGCAGTGATGATACCAGAAATAGCACTGAGAACATAGAGGGAATGAAAAATTGCTCTTTCAATGCAACC
ACAGTAGTAAGAGATAAGCAGCAGAAAGTGTCTGCACTTTTTTATAGACTTGATATAGTACCACTTACTGAGAAGGAAT
CTAGTGAGAACTCTAGTGGGTATTATAGATTAATAAATTGTAATACCTCAGCCATAACACAAGCCTGTCCAAAGGTTAC
TTTTGATCCAATTCCTATACACTATTGTACTCCAGCTGGTTATGCAATTCTAAAGTGTAATAATAAGACATTCAATGGG
ACAGGGCCATGCTCTAATGTTAGCACAGTACAATGTACACATGGGATTAAGCCAGTGGTATCAACTCAACTACTGTTAA
ATGGTAGCCTATCAGAAGGAGGAATAATAATTAGATCTGAAAATCTGACAGACAATGTCAAAACAATAATAGTACATCT
TAATGAATCTGTAGGAATTGTATGTACAAGACCCAACAATAATACAAGAAAAAGTATAAGGATAGGACCAGGACAAACA
TTCTATGCAACAGGAGAAATAATAGGAGACATAAGACAAGCACATTGTAACATTAGTGAATATAAATGGAATAAAACTT
TGCAAAGGGTAAGTGAAAAATTAGCAGAATACTTCCCGAATGACACAATAAAATTTGCACCATCCTCAGGAGGGGACCT
AGAAATTACAACACATAGCTTTAATTGTAGAGGAGAATTTTTCTATTGTAATACATCAGGCCTGTTTAATGGTACATAT
AATGGTACATACAATGCTAATGGTACAGAAAACGCAACCATCACAATCCCATGCAGAATAAAGCAAATTATAAATATGT
GGCAGGAGGTAGGAAGAGCAATGTATGCCCCTCCCATTGCAGGAAACATAACATGTAACTCAAGTATCACAGGACTACT
ATTGGTACGTGATGGAGGAATAAATAATACAAATAATACAGAGACATTCAGACCTGGAGGAGGAGATATGAAGGACAAT
TGGAGAAGTGAATTATACAAATATAAAGTAGTAGAAGTTAAACCATTGGGAGTAGCACCCACTACAGCAAAAAGGAGAG
TGGTGGAGAGAGAAAAAAGGGGAATAGGAATAGGAGCTGTGTTCCTTGGGTTCTTGGGAGTAGCAGGAAGCACTATGGG
CGCGGCGTCTGTAACGCTGACGGTACAGGCCAGACAATTGCTGTCTGGTATAGTGCAACAGCAAAGCAATTTGCTGAAG
GCTATAGAGGCGCAACAGCATCTGTTGCAACTCACGGTCTGGGGCATTAAGCAGCTCCAGACAAGAGTCCTGGCTATAG
AAAGATACCTAAAGGATCAACAGCTCCTAGGGATTTGGGGTTGCTCTGGAAAAATCATCTGCACCACTGCTGTACCTTG
GAACTCCAGTTGGAGTAACAAGTCTCAACAAGATATTTGGGATAACATGACCTGGATGCAATGGGATAAAGAAATTAGT
AATTACACAAACACAATATACAGTTTGCTTGAAGAATCGCAAATCCAGCAGGAGAAGAATGAAAAAGATCTATTAGCAT
TGGACAGCTGGAAAAATCTATGGAATTGGTTTAACATATCAAATTGGCTGTGGTATATAAAAATATTCATAATAATAGT
AGGAAGCTTGATAGGTTTAAGAATAATTTTTGCTGTGCTCTCTATAGTGAATAGAGTTAGGCAGGGATACTCACCTTTG
TCGTTTCAGATCCTTACCCCGAACCCAGGGGGACCCGACAGGCTCGGAAGAATCGAAGAAGAAGGTGGAGAGCAAGACA
AAGGAAGATCCATTCGATTAGTGAACGGATTCTTTGCGCTTGCCTGGGACGACCTGCGGAACCTGTGCCTCTTCAGCTA
CCACCGCTTGAGAGACTTCATATTAGTGGCAGCGAGAGTGGTGGAACTTCTGGGACGCAGCAGTCTCAGGGGACTACAG
AGGGGGTGGGAAGCCCTTAAATATCTGGGAAGTCTTGTGCAGTACTGGGGTCAGGAGCTAAAAAAGAGTGCTATTAGTC
TGTTTGATGCCATAGCAATAGCAGTAGCTGAAGGAACAGATAGGATTATA{{Team:NYMU-Taipei/BBCut|GAATTC}}ATACAAAGATTTTGTAGAGCTAT
CTACAACATACCTAGAAGAATAAGACAGGGATTTGAAGCAGCTTTGCAATAA|HIV-1 gp120 original|2580}}

{{:Team:NYMU-Taipei/Seq|{{Team:NYMU-Taipei/Primer|ATGAGAGTGACGGGGATC|f primer}}AGGAAGAATTATCGGCACTTATGGAAATGGGGCACCATGCTCCTTGGAATGTTGATGATCT
GTAGTGCTGTAGAAAACTTGTGGGTCACAGTCTATTATGGGGTACCTATATGGAAAGATGCAACCACCACTTTATTTTG
TGCATCAGATGCTAGAGCATATGATACAGAGGTACATAATGTTTGGGCTACACATGCCTGTGTACCCACAGACCCCAAC
CCACAAGAACTGGTTTTGGTAAATGTAACAGAAAATTTTAACATGTGGAAAAATGACATGGTAGATCAGATGCATGAAG
ATGTAATCAGTTTATGGGATCAAAGCCTAAAGCCATGTGTAAAGTTGACCCCACTCTGTGTCACTTTAAACTGTACAAA
TGCTACCCGTAGCAGTGATGATACCAGAAATAGCACTGAGAACATAGAGGGAATGAAAAATTGCTCTTTCAATGCAACC
ACAGTAGTAAGAGATAAGCAGCAGAAAGTGTCTGCACTTTTTTATAGACTTGATATAGTACCACTTACTGAGAAGGAAT
CTAGTGAGAACTCTAGTGGGTATTATAGATTAATAAATTGTAATACCTCAGCCATAACACAAGCCTGTCCAAAGGTTAC
TTTTGATCCAATTCCTATACACTATTGTACTCCAGCTGGTTATGCAATTCTAAAGTGTAATAATAAGACATTCAATGGG
ACAGGGCCATGCTCTAATGTTAGCACAGTACAATGTACACATGGGATTAAGCCAGTGGTATCAACTCAACTACTGTTAA
ATGGTAGCCTATCAGAAGGAGGAATAATAATTAGATCTGAAAATCTGACAGACAATGTCAAAACAATAATAGTACATCT
TAATGAATCTGTAGGAATTGTATGTACAAGACCCAACAATAATACAAGAAAAAGTATAAGGATAGGACCAGGACAAACA
TTCTATGCAACAGGAGAAATAATAGGAGACATAAGACAAGCACATTGTAACATTAGTGAATATAAATGGAATAAAACTT
TGCAAAGGGTAAGTGAAAAATTAGCAGAATACTTCCCGAATGACACAATAAAATTTGCACCATCCTCAGGAGGGGACCT
AGAAATTACAACACATAGCTTTAATTGTAGAGGAGAATTTTTCTATTGTAATACATCAGGCCTGTTTAATGGTACATAT
AATGGTACATACAATGCTAATGGTACAGAAAACGCAACCATCACAATCCCATGCAGAATAAAGCAAATTATAAATATGT
GGCAGGAGGTAGGAAGAGCAATGTATGCCCCTCCCATTGCAGGAAACATAACATGTAACTCAAGTATCACAGGACTACT
ATTGGTACGTGATGGAGGAATAAATAATACAAATAATACAGAGACATTCAGACCTGGAGGAGGAGATATGAAGGACAAT
TGGAGAAGTGAATTATACAAATATAAAGTAGTAGAAGTTAAACCATTGGGAGTAGCACCCACTACAGCAAAAAGGAGAG
TGGTGGAGAGAGAAAAAAGGGGAATAGGAATAGGAGCTGTGTTCCTTGGGTTCTTGGGAGTAGCAGGAAGCACTATGGG
CGCGGCGTCTGTAACGCTGACGGTACAGGCCAGACAATTGCTGTCTGGTATAGTGCAACAGCAAAGCAATTTGCTGAAG
GCTATAGAGGCGCAACAGCATCTGTTGCAACTCACGGTCTGGGGCATTAAGCAGCTCCAGACAAGAGTCCTGGCTATAG
AAAGATACCTAAAGGATCAACAGCTCCTAGGGATTTGGGGTTGCTCTGGAAAAATCATCTGCACCACTGCTGTACCTTG
GAACTCCAGTTGGAGTAACAAGTCTCAACAAGATATTTGGGATAACATGACCTGGATGCAATGGGATAAAGAAATTAGT
AATTACACAAACACAATATACAGTTTGCTTGAAGAATCGCAAATCCAGCAGGAGAAGAATGAAAAAGATCTATTAGCAT
TGGACAGCTGGAAAAATCTATGGAATTGGTTTAACATATCAAATTGGCTGTGGTATATAAAAATATTCATAATAATAGT
AGGAAGCTTGATAGGTTTAAGAATAATTTTTGCTGTGCTCTCTATAGTGAATAGAGTTAGGCAGGGATACTCACCTTTG
TCGTTTCAGATCCTTACCCCGAACCCAGGGGGACCCGACAGGCTCGGAAGAATCGAAGAAGAAGGTGGAGAGCAAGACA
AAGGAAGATCCATTCGATTAGTGAACGGATTCTTTGCGCTTGCCTGGGACGACCTGCGGAACCTGTGCCTCTTCAGCTA
CCACCGCTTGAGAGACTTCATATTAGTGGCAGCGAGAGTGGTGGAACTTCTGGGACGCAGCAGTCTCAGGGGACTACAG
AGGGGGTGGGAAGCCCTTAAATATCTGGGAAGTCTTGTGCAGTACTGGGGTCAGGAGCTAAAAAAGAGTGCTATTAGTC
TGTTTGATGCCATAGCAATAGCAGTAGCTGAAGGAACA{{Team:NYMU-Taipei/Primer|GATAGGATTATA{{Team:NYMU-Taipei/BBCut|GAgTTC|mut}}ATACAAAGATTTTG|primer2}}TAGAGCTAT
CTACAACATACCTAGAAGAATAAGACAGG{{Team:NYMU-Taipei/Primer|GATTTGAAGCAGCTTTGCAATAA|r primer}}|HIV-1 gp120 original|2580}}

outer primer:
 gaattcgcggccgcttctag ATGAGAGTGACGGGGATC 54deg, GC:56%, 20+18=38bp
 ctgcagcggccgctactagta TTATTGCAAAGCTGCTTCAAATC 54deg, GC:35%, 21+23=44bp
inner primer: EcoRI@pos2500, 54deg, GC:28%, 32bp
 GATAGGATTATAGAgTTCATACAAAGATTTTG 
 CAAAATCTTTGTATGAAcTCTATAATCCTATC
===adenovirus type 13 penton base===
*the same as type 3
        1 atgaggcgcg cggtggtgtc ttcctctcct cctccctcgt acgagagcgt gatggcgcag
       61 gcgaccctgg aggttccgtt tgtgcctccg cggtatatgg ctcctacgga gggcagaaac
      121 agcattcgtt actcggagct ggctccgcag tacgacacca ctcgcgtgta cttggtggac
      181 aacaagtcgg cggacatcgc ttccctgaac taccaaaacg accacagcaa cttcctgacc
      241 acggtggtgc agaacaacga tttcaccccc gccgaggcca gcacgcagac gataaatttt
      301 gacgagcggt cgcggtgggg cggtgatctg aagaccattc tgcacaccaa catgcccaat
      361 gtgaacgagt acatgttcac cagcaagttt aaggcgcggg tgatggtggc taggaagcac
      421 cccaaagatg tgcaggatcc taatgataga agcaaggata tcttagagta taagtggttt
      481 gagtttaccc tgcccgaggg caacttttcc gagaccatga ccatagacct gatgaacaac
      541 gccatcttgg aaaactactt gcaagtgggg cggcaaaatg gcgtgctgga gagtgatatc
      601 ggagtcaagt ttgacagcag gaatttcaga ctgggctggg acccggagac caagctggta
      661 atgccggggg tctacaccta cgaggccttc cacccggacg tggtgctact gccgggctgc
      721 ggggtggact tcaccgagag ccgcctgagc aacctcctgg gcattcgcaa gaagcaacct
      781 tttcaagagg gcttcagaat catgtatgag gatctagaag ggggcaacat ccctgccctg
      841 ctggatgtgg atgcatacct caaaagcaag aatgatctgg aagaggctac caaaaatgca
      901 aacagagctg ctgctaatgg aggtggtgaa actaggggag atacttttct caccaccgaa
      961 cagctgagag ccgctggcaa ggagctggtt attaagccaa tcaaggaaga tgctagcaaa
     1021 aggagctata atgtcataga tgacacccat gacacgctgt accgaagctg gtacctgtcc
     1081 tacacatacg gggaccccga gaagggagtg cagtcgtgga cgctgctcac caccccggac
     1141 gtcacctgcg gcgcggagca agtctactgg tcgctgccgg acctcatgca agaccccgtc
     1201 accttccgct ctacccagca agtcagcaac taccccgtgg tcggcgccga gctcatgcct
     1261 ttccgcgcca agagctttta caacgacctc gccgtctact cccagctcat ccgcagctac
     1321 acctccctca cccacgtctt caaccgcttc cccgacaacc agatcctctg ccgcccgccc
     1381 gcgcccacca tcaccaccgt cagtgaaaac gtgcctgctc tcacagatca cgggacgcta
     1441 ccgctgcgca gcagtatccg cggagtccag cgagtgaccg tcactgacgc ccgtcgccgc
     1501 acctgtccct acgtctacaa ggccctgggc atagtcgcgc cgcgcgtgct ctctagtcgc
     1561 accttt

===adenovirus type 13 fiber protein===
        1 ctttggacaa cacctgatcc atcgcccaat tgtaaagccg aaacagaaaa ggactcaaag
       61 cttactttgg ttctaacaaa atgcggaagt caaatcttag ctactgtgtc tattattgtt
      121 cttaagggta aatatgaatt tgttaaaaaa gaaacagagc ctaagtcctt tgatgttaaa
      181 ttgctgtttg acagcaaagg agtgcttcta ccaacttcaa atctttccaa agaatattgg
      241 aactatagaa gttatgataa caacatagga actccttatg aaaatgcagt tccatttatg
      301 ccaaatctaa aagcttatcc aaaaccaact aaaactgctt cagataaagc agaaaataaa
      361 ataagcagtg ctaaaaacaa aattgtaagc aacttctact ttggagggca agcatatcaa
      421 cctgggacaa taataattaa gtttaatgag gaaatagatg agacatgtgc atactctatc
      481 acctttaact ttggttgggg caaggtatat gacaatccat ttccatttga taccacctct
      541 tttaccttct cttatattgc ccaagaaaat gaagacaaag actaa
*primer for adenovirus penton base and fiber protein link
F:gcttctag atgaggcgcgcgg 55deg,77%GC, 8+13bp<br>
R:ctgcagcggccgctactagta ttagtctttgtcttcattttcttggg 55deg, 35%GC 21+26bp