Team:Washington/Notebook/Microscope

From 2009.igem.org

(Difference between revisions)
(New page: ==== Microscope ==== # Set up overnights of parts 48-51. Let grow overnight. # Dilute 1 ul overnight into 1ml # Add 1 mm IPTG and let grow for four hours # After cells have grown up, place...)
Line 1: Line 1:
 +
__NOTOC__
 +
{{Template:Team:Washington/Templates/Header}}
 +
==== Microscope ====
==== Microscope ====
# Set up overnights of parts 48-51. Let grow overnight.
# Set up overnights of parts 48-51. Let grow overnight.

Revision as of 05:07, 15 October 2009

Uw title logo.png

Microscope

  1. Set up overnights of parts 48-51. Let grow overnight.
  2. Dilute 1 ul overnight into 1ml
  3. Add 1 mm IPTG and let grow for four hours
  4. After cells have grown up, place in flourophore (1 um) and allow time to bind (1 hour)
  5. Also place 1 ul beads in 1 ml along with 1 ul flourophore.
  6. Allow beads to bind to flourophore, then spin beads down, remove supernatent and replace with 1 ml water.
  7. Next place the cells and the beads under the microscope
Retrieved from "http://2009.igem.org/Team:Washington/Notebook/Microscope"