"
Team:DTU Denmark/parts
From 2009.igem.org
| Line 202: | Line 202: | ||
<p align="justify">This variant of biobrick BBa_K194001 has been destabilized by fusing biobrick BBa_K194000. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes.</p> | <p align="justify">This variant of biobrick BBa_K194001 has been destabilized by fusing biobrick BBa_K194000. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes.</p> | ||
Registry id: <a href="http://partsregistry.org/Part:BBa_K194002" target="_blank">BBa_K194002</a> | Registry id: <a href="http://partsregistry.org/Part:BBa_K194002" target="_blank">BBa_K194002</a> | ||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | <font size="3"><b>USER cassette for insertion of USER fragments</b></font><br> | ||
| + | |||
| + | <p align="justify">This biobrick contains a PacI/Nt.BbvCI and a AsiSI/Nb.BtsI USER cassette for insertion of PCR fragments using the USER cloning standard. PCR fragments containing uracil is treated with a uracil DNA glycosylase that removes the uracil exposing a predesigned 8bp overhang allowing for cloning without the need for ligase. </p> | ||
| + | Registry id: <a href="http://partsregistry.org/Part:BBa_K194003" target="_blank">BBa_K194003</a> | ||
<br> | <br> | ||
<br> | <br> | ||
| Line 215: | Line 222: | ||
<!-- INSERT GREY BOX TEXT HERE! (formatting: <b>bold</> <i>italic> <h4>header</h4>) --> | <!-- INSERT GREY BOX TEXT HERE! (formatting: <b>bold</> <i>italic> <h4>header</h4>) --> | ||
| - | <p | + | <p>No facts</p> |
Revision as of 12:26, 15 October 2009
| Home | The Team | The Project | Parts submitted | Modelling | Notebook |
|
View all parts in registry Parts registry |
Parts submitted to registry cln2 PEST destabilization domain for rapid protein turnover C-terminal domain of Saccharomyces cerevisiae cyclin 2 (CLN2) gene. It has been shown that this region of the protein, which is rich of PEST motifs, leads to a destabilization of the protein. Hence this Tag can be used to increase the turn-over rate of a protein. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues changes the half-life of a GFP from 7h and down to 30 minutes. Registry id: BBa_K194000GFP (a yeast- and FACS-optimized GFP variant) Green Fluorescent Protein (GFP) codonoptimized for yeast. Registry id: BBa_K194001Destabilized GFP for yeast This variant of biobrick BBa_K194001 has been destabilized by fusing biobrick BBa_K194000. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes. Registry id: BBa_K194002USER cassette for insertion of USER fragments This biobrick contains a PacI/Nt.BbvCI and a AsiSI/Nb.BtsI USER cassette for insertion of PCR fragments using the USER cloning standard. PCR fragments containing uracil is treated with a uracil DNA glycosylase that removes the uracil exposing a predesigned 8bp overhang allowing for cloning without the need for ligase. Registry id: BBa_K194003 |
Facts about parts No facts |
| Comments or questions to the team? Please Email us -- Comments of questions to webmaster? Please Email us |
