Team:UNIPV-Pavia/Notebook/Week3Jun

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= Week from June 15th, to June 21st, 2009 =
= Week from June 15th, to June 21st, 2009 =
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Jun">Previous Week</a> |
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Jun">Next Week</a>
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== <html><font class="dayw_style">June, 15th</font></html> ==
== <html><font class="dayw_style">June, 15th</font></html> ==
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Jun">Previous Week</a> |
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Jun">Next Week</a>
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Revision as of 12:38, 21 June 2009

EthanolPVanimation.gif

December 2008
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April 2009
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July 2009
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Week from June 15th, to June 21st, 2009

Previous Week | Next Week

June, 15th

Top

June, 16th

Top

June, 17th

Trasformation of Ligations

A5: BBa_Q0040 with BBa_I732017 (RBS-TETR-PTET-RBS-LACZ-TT)
A6: BBa_J23100 with BBa_Q0040 (PCON-RBS-TETR-TT-PTET )

  • 1 ul A5 in Top10 Competent
  • 1 ul A6 in Top10 Competent

Mix 80 ul of SOC with 20 ul X-GAL (40 mg/ml) and infect LB+Amp Agar plates.

  • Add 250 ul SOC in 50 ul A5+ Top10
  • Add 250 ul SOC in 50 ul A6+ Top10

1 hr incubation at 37°C (falcons with shaking 220 rpm)

Infect:

  1. X-GAL plate with 200 ul A5
  2. X-GAL plate with 100 ul A5
  3. Agar plate with 200 ul A6
  4. X-GAL plate with 20 ul of BBa_E0240 from glycerol stock (negative control).

Expected results:

  • Plates 1 and 2 should develop blue colonies
  • Plate 4 should remain colorless

16 hr incubation at 37°C.

Top

June, 18th

Experiment with Tecan F200

  • Description
  • Purpose:
  • Materials & Methods
  • Protocol
  • Results

Top

June, 19th

Top

June, 20th

Top

June, 21st

Top

Previous Week | Next Week

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