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HKU-HKBU/29 July 2009
From 2009.igem.org
(Difference between revisions)
(New page: =====Co-transformation Transform two plasmids (A+, C+) to MG3. ① Plac-CheZ-His-TT C+ ② Lac Iq A+===== =====14:10 Subculture to warm room (37℃), shaking for 1.5h.===== =====15:40...) |
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| - | =====Co-transformation | + | {{Team:HKU-HKBU/style.css}} |
| - | Transform two plasmids (A+, C+) to MG3. | + | {{Team:HKU-HKBU/script.js}} |
| - | ① Plac-CheZ-His-TT C+ | + | {{Team:HKU-HKBU/header}} |
| - | ② Lac Iq A+===== | + | |
| + | |||
| + | |||
| + | =====Co-transformation Transform two plasmids (A+, C+) to MG3.① Plac-CheZ-His-TT C+② Lac Iq A+===== | ||
=====14:10 Subculture to warm room (37℃), shaking for 1.5h.===== | =====14:10 Subculture to warm room (37℃), shaking for 1.5h.===== | ||
| - | =====15:40 take the subculture bacteria out from the warm room. | + | =====15:40 take the subculture bacteria out from the warm room.Freeze the bacteria in 0℃ ice water for 15min. |
| - | Freeze the bacteria in 0℃ ice water for 15min. | + | Centrifuge in 4000rmp at 0℃ for 2min.Decent supernatant===== |
| - | Centrifuge in 4000rmp at 0℃ for 2min. | + | |
| - | Decent supernatant===== | + | |
Revision as of 14:28, 16 October 2009
Co-transformation Transform two plasmids (A+, C+) to MG3.① Plac-CheZ-His-TT C+② Lac Iq A+
14:10 Subculture to warm room (37℃), shaking for 1.5h.
=====15:40 take the subculture bacteria out from the warm room.Freeze the bacteria in 0℃ ice water for 15min. Centrifuge in 4000rmp at 0℃ for 2min.Decent supernatant=====
