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HKU-HKBU/29 July 2009
From 2009.igem.org
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=====Co-transformation Transform two plasmids (A+, C+) to MG3.① Plac-CheZ-His-TT C+② Lac Iq A+===== | =====Co-transformation Transform two plasmids (A+, C+) to MG3.① Plac-CheZ-His-TT C+② Lac Iq A+===== | ||
=====14:10 Subculture to warm room (37℃), shaking for 1.5h.===== | =====14:10 Subculture to warm room (37℃), shaking for 1.5h.===== | ||
| - | =====15:40 take the subculture bacteria out from the warm room.Freeze the bacteria in 0℃ ice water for 15min. | + | =====15:40 take the subculture bacteria out from the warm room.Freeze the bacteria in 0℃ ice water for===== |
| - | Centrifuge in 4000rmp at 0℃ for 2min.Decent supernatant===== | + | =====15min.===== |
| + | =====Centrifuge in 4000rmp at 0℃ for 2min.Decent supernatant===== | ||
Revision as of 14:29, 16 October 2009
