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HKU-HKBU/29 July 2009
From 2009.igem.org
(Difference between revisions)
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Co-transformation Transform two plasmids (A+, C+) to MG3.① Plac-CheZ-His-TT C+② Lac Iq A+ | Co-transformation Transform two plasmids (A+, C+) to MG3.① Plac-CheZ-His-TT C+② Lac Iq A+ | ||
| - | 14:10 Subculture to warm room (37℃), shaking for 1.5h. | + | =====14:10 Subculture to warm room (37℃), shaking for 1.5h.===== |
| - | 15:40 take the subculture bacteria out from the warm room.Freeze the bacteria in 0℃ ice water for | + | |
| - | 15min. | + | =====15:40 take the subculture bacteria out from the warm room.Freeze the bacteria in 0℃ ice water for |
| - | Centrifuge in 4000rmp at 0℃ for 2min.Decent supernatant | + | 15min.Centrifuge in 4000rmp at 0℃ for 2min.Decent supernatant===== |
Revision as of 14:30, 16 October 2009
Co-transformation Transform two plasmids (A+, C+) to MG3.① Plac-CheZ-His-TT C+② Lac Iq A+
14:10 Subculture to warm room (37℃), shaking for 1.5h.
=====15:40 take the subculture bacteria out from the warm room.Freeze the bacteria in 0℃ ice water for 15min.Centrifuge in 4000rmp at 0℃ for 2min.Decent supernatant=====
