"
Team:Paris/25 August 2009
From 2009.igem.org
(Difference between revisions)
(New page: {{Template:Paris2009}} {{Template:Paris2009_menu}} ==NoteBook== {| style="color:#1b2c8a;background-color:#0;" cellpadding="0" cellspacing="1" border="0" bordercolor="#fff" width="100%" a...) |
(→Lab work) |
||
| (21 intermediate revisions not shown) | |||
| Line 11: | Line 11: | ||
!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=10 }} | !align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=10 }} | ||
|} | |} | ||
| + | |||
| + | {{Paris2009_Calendar_Link|24_August_2009|26_August_2009}} | ||
| + | <center> '''August 25th''' </center> | ||
<html> | <html> | ||
| Line 19: | Line 22: | ||
</html> | </html> | ||
| + | ==Lab work== | ||
| + | <div class="charlotte"> | ||
| + | Digestion/Gel purification w/ Soufifi | ||
| + | </div> | ||
| + | <div class="experience"> | ||
| - | |||
| + | Digestion of OmpA Linker by SacI and Spe I | ||
| - | + | Digestion of Ompa Signal by the same enzymes | |
| - | < | + | <center> [[Image:KR000778.PNG]] </center> |
</div> | </div> | ||
| - | <div | + | |
| + | |||
| + | |||
| + | <div class="caroline"> | ||
| + | A41 OmpAsignal | ||
</div> | </div> | ||
| - | <div | + | <div class="experience"> |
| - | + | PCR OmpAsignal A41 ; 70,4° ; 9s ; matrice:pINK2 | |
| + | Gel purif | ||
| + | </div> | ||
| + | <div class="caroline"> | ||
| + | A41 OmpAsignal A40 TolRII | ||
| + | </div> | ||
| + | <div class="experience"> | ||
| + | Digestion X/P | ||
| - | + | Ligation pSB1A3 | |
| - | + | A41 OmpAsignal ligation topovector | |
| + | </div> | ||
| + | <div class="vicard"> | ||
| + | PCR+ check + purification | ||
| + | </div> | ||
| + | <div class="experience"> | ||
| + | |||
| + | |||
| + | PCR->A34 (NTER ClyA) | ||
| + | |||
| + | Check gel--> OK | ||
| + | |||
| + | Purification-->ok | ||
| + | <center> [[Image:Nter_clyA_25aout.JPG]] </center> | ||
| - | |||
</div> | </div> | ||
| - | <div | + | |
| + | <div class="vicard"> | ||
| + | Miniprep+Sequencing | ||
</div> | </div> | ||
| - | <div | + | <div class="experience"> |
| - | + | ||
| + | Miniprep of all colonie of nter RFP and Cter RFP | ||
| - | + | Colonie DO: | |
| - | + | Nter RFP: for colonie | |
| + | |||
| + | -1:1,80µg/ml | ||
| + | |||
| + | (Miniprep: 3,33µL+ oligos VF2(1/50): 2,45µL+ H20: 9,22µL) | ||
| + | |||
| + | (Miniprep: 3,33µL+ oligos VR(1/50): 2,45µL+ H20: 9,22µL) | ||
| + | |||
| + | -2:1,90µg/ml | ||
| + | |||
| + | (Miniprep: 3,16µL+ oligos VF2(1/50): 2,45µL+ H20: 9,39µL) | ||
| + | |||
| + | (Miniprep: 3,16µL+ oligos VR(1/50): 2,45µL+ H20: 9,39µL) | ||
| + | |||
| + | -3:1,65µg/ml | ||
| + | |||
| + | (Miniprep: 3,64µL+ oligos VF2(1/50): 2,45µL+ H20: 8,81µL) | ||
| + | |||
| + | |||
| + | (Miniprep: 3,64µL+ oligos VR(1/50): 2,45µL+ H20: 8,81µL) | ||
| + | |||
| + | -4:1,66µg/ml | ||
| + | |||
| + | |||
| + | (Miniprep: 3,61µL+ oligos VF2(1/50): 2,45µL+ H20: 8,94µL) | ||
| + | |||
| + | (Miniprep: 3,61µL+ oligos VR(1/50): 2,45µL+ H20: 8,94µL) | ||
| + | |||
| + | Cter RFP: for colonie | ||
| + | |||
| + | -1:1,80µg/ml | ||
| + | |||
| + | -2:1,90µg/ml | ||
| + | |||
| + | -3:1,70µg/ml | ||
| + | |||
| + | -4:1,60µg/ml | ||
| + | |||
| + | </div> | ||
Latest revision as of 23:20, 16 October 2009
NoteBook
|
|
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Lab work
Digestion/Gel purification w/ Soufifi
Digestion of OmpA Linker by SacI and Spe I
Digestion of Ompa Signal by the same enzymes
A41 OmpAsignal
PCR OmpAsignal A41 ; 70,4° ; 9s ; matrice:pINK2
Gel purif
A41 OmpAsignal A40 TolRII
Digestion X/P
Ligation pSB1A3
A41 OmpAsignal ligation topovector
PCR+ check + purification
PCR->A34 (NTER ClyA)
Check gel--> OK
Purification-->ok
Miniprep+Sequencing
Miniprep of all colonie of nter RFP and Cter RFP
Colonie DO:
Nter RFP: for colonie
-1:1,80µg/ml
(Miniprep: 3,33µL+ oligos VF2(1/50): 2,45µL+ H20: 9,22µL)
(Miniprep: 3,33µL+ oligos VR(1/50): 2,45µL+ H20: 9,22µL)
-2:1,90µg/ml
(Miniprep: 3,16µL+ oligos VF2(1/50): 2,45µL+ H20: 9,39µL)
(Miniprep: 3,16µL+ oligos VR(1/50): 2,45µL+ H20: 9,39µL)
-3:1,65µg/ml
(Miniprep: 3,64µL+ oligos VF2(1/50): 2,45µL+ H20: 8,81µL)
(Miniprep: 3,64µL+ oligos VR(1/50): 2,45µL+ H20: 8,81µL)
-4:1,66µg/ml
(Miniprep: 3,61µL+ oligos VF2(1/50): 2,45µL+ H20: 8,94µL)
(Miniprep: 3,61µL+ oligos VR(1/50): 2,45µL+ H20: 8,94µL)
Cter RFP: for colonie
-1:1,80µg/ml
-2:1,90µg/ml
-3:1,70µg/ml
-4:1,60µg/ml
