Team:HKUST/OdorantSensoring

From 2009.igem.org

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In this sub-project, we are constructing a chimeric G protein-coupled receptor (GPCR) to be functionally expressed in the heterologous yeast system. The yeast pheromone sensing pathway is used and adapted to express our odorant receptor. We have demonstrated that this engineered receptor could response to a volatile molecule, and in principle showed that this method could potentially be applied to other insect odorant receptors.  
In this sub-project, we are constructing a chimeric G protein-coupled receptor (GPCR) to be functionally expressed in the heterologous yeast system. The yeast pheromone sensing pathway is used and adapted to express our odorant receptor. We have demonstrated that this engineered receptor could response to a volatile molecule, and in principle showed that this method could potentially be applied to other insect odorant receptors.  
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<li><a href="https://2009.igem.org/Team:HKUST/Back1">Background</a></li>
<li><a href="https://2009.igem.org/Team:HKUST/Back1">Background</a></li>
<li><a href="https://2009.igem.org/Team:HKUST/Group1">Experimental design</a></li>
<li><a href="https://2009.igem.org/Team:HKUST/Group1">Experimental design</a></li>

Revision as of 11:07, 17 October 2009

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Welcome

In this sub-project, we are constructing a chimeric G protein-coupled receptor (GPCR) to be functionally expressed in the heterologous yeast system. The yeast pheromone sensing pathway is used and adapted to express our odorant receptor. We have demonstrated that this engineered receptor could response to a volatile molecule, and in principle showed that this method could potentially be applied to other insect odorant receptors.

  • Background
  • Experimental design
  • Parts design
  • Experimental result
  • Future work
  • Reference
  • HKUST