Team:Freiburg bioware/cloning1

From 2009.igem.org

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  class="t">The Team</span></a>
  class="t">The Team</span></a>
     <ul>
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       <li><a href="#">Overview</a></li>
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       <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team">Overview</a></li>
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       <li><a href="#">Photos</a></li>
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       <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team/Portraits">Portraits</a></li>
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     </ul></li>
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  href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice"><span
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  href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice" class="active"><span
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  class="r"></span><span class="t">Notebook</span></a></li>
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  href="https://2009.igem.org/Team:Freiburg_bioware/cloning" class="active"><span
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  href="https://2009.igem.org/Team:Freiburg_bioware/cloning"><span
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  class="t">Parts</span></a></li>
  class="t">Parts</span></a></li>
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<h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> &nbsp;Blank<span
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<h3>Index</h3><br>
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*<a href="#2">His+FluA+Split+Foki_pMA
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*<a href="#19">
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His+FluA+longlinker+Foki_pMA
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</div><a name="35"></a>
 
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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
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<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
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<img src="https://static.igem.org/mediawiki/2009/thumb/d/db/Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.jpg" name="Strep+FluA+shortlinker+FokA in pMA Vector" width="480" height="480" />
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<br><p align="center">Strep+FluA+shortlinker+FokA in pMA Vector</p>
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nucleotid sequence of ORF-1:<br>
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ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
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CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
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AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
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AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
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GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
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GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
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CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
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CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
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AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
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ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
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GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
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AAGTGAACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAG
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GAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTA
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TATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGG
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AAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAA
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CATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTC
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CCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTT
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ATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAG
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AACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTA
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TCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTA
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AAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGC
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AATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGAT
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CAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATG
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<h4>AA sequence of ORF-1:<br></h4>
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MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
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TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
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NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
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AACKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEF
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FMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKE
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NQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEEL
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LIGGEMIKAGTLTLEEVRRKFNNGEINFTG
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<h4>Commented GenBank file</h4>
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<li> <a href="https://static.igem.org/mediawiki/2009/4/49/Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.txt">Strep+FluA+shortlinker+FokA_pMA.txt</a></li>
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Revision as of 12:48, 17 October 2009

FREiGEM