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Team:DTU Denmark
From 2009.igem.org
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<p align="justify">Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TM) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions.</p> | <p align="justify">Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TM) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions.</p> | ||
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| + | <font size="3"><b>The USER<sup>TM</sup> fusion primer design softwars</b></font><br> | ||
| + | <a href="https://2009.igem.org/Team:DTU_Denmark/USERprogram" CLASS=leftbar>Design your primers here</a><br><br> | ||
Revision as of 13:35, 17 October 2009
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DTU Denmark Project abstract The Redoxilator By in silico design and computer modelling followed by gene synthesis, we have constructed a molecular NAD/NADH ratio sensing system in Saccharomyces cerevisiae. The sensor works as an inducible transcription factor being active only at certain levels of the NAD/NADH ratios. By the coupling of a yeast optimized fast degradable GFP, the system can be used for in vivo monitoring of NAD/NADH redox poise. A future novel application of the system is heterologous redox coupled protein production in yeast. 
The USER fusion standard Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TM) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions. The USERTM fusion primer design softwarsDesign your primers here |
NEWS NEWS NEWS NEWS 28.09.2009 The wiki page layout is now settled. Stay tuned for project results!
We just received word that Lundbeckfonden has granted us $8000 USD for completion of our project! We owe them our gratitude, and continue our work delighted that thanks to Lundbeckfonden, Otto Mønsteds Fond, and Center for Microbial Biotechnology, we are now 100% funded and ready for iGEM 2009!
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