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Team:Wisconsin-Madison/Notebook Protocols
From 2009.igem.org
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| + | Plasmid Preparation by Alkaline Lysis | ||
| + | Plasmid Preparation by Boiling Lysis | ||
| + | Transformation of Plasmids into Competant Cells | ||
| + | Preparation of Competent Cells | ||
| + | Cloning Protocols | ||
| + | Polymerase Chain Reaction (PCR) | ||
| + | Ligation | ||
| + | Digestion | ||
| + | Inoculation of Plasmids in Liquid Media | ||
| + | Inoculation of Cyanobacteria | ||
| + | Loading Gel Protocol | ||
| + | Plasmid Clean-up | ||
| + | Gel Extraction from Agar | ||
| + | Sequencing Reaction | ||
| + | Colony Polymerase Chain Reaction (PCR) | ||
Revision as of 20:10, 17 October 2009
Protocols
Plasmid Preparation by Alkaline Lysis
Plasmid Preparation by Boiling Lysis
Transformation of Plasmids into Competant Cells
Preparation of Competent Cells
Cloning Protocols
Polymerase Chain Reaction (PCR)
Ligation
Digestion
Inoculation of Plasmids in Liquid Media
Inoculation of Cyanobacteria
Loading Gel Protocol
Plasmid Clean-up
Gel Extraction from Agar
Sequencing Reaction
Colony Polymerase Chain Reaction (PCR)
