Team:Wisconsin-Madison/Notebook Protocols

From 2009.igem.org

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Revision as of 20:16, 17 October 2009



PROTOCOLS
  • Plasmid Preparation by Alkaline Lysis
  • Plasmid Preparation by Boiling Lysis
  • Restriction Digestion
  • Transformation of Plasmids into Competant Cells
  • Preparation of Competent Cells
  • Cloning Protocols
  • Polymerase Chain Reaction (PCR)
  • Ligation
  • Digestion - Cloning
  • Inoculation of Plasmids in Liquid Media
  • Inoculation of Cyanobacteria
  • Loading Gel Protocol
  • Plasmid Clean-up
  • Gel Extraction from Agar
  • Sequencing Reaction
  • Colony Polymerase Chain Reaction (PCR)
  • Genomic DNA Extraction
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