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Team:DTU Denmark/parts
From 2009.igem.org
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<font size="3"><b>USER cassette for insertion of USER fragments</b></font><br> | <font size="3"><b>USER cassette for insertion of USER fragments</b></font><br> | ||
| - | <p align="justify">This biobrick contains a PacI/Nt.BbvCI | + | <p align="justify">This biobrick contains a PacI/Nt.BbvCI USER cassette for insertion of PCR fragments using the USER cloning standard. PCR fragments containing uracil is treated with a uracil DNA glycosylase that removes the uracil exposing a predesigned 8bp overhang allowing for cloning without the need for ligase. </p> |
Registry id: <a href="http://partsregistry.org/Part:BBa_K194003" target="_blank">BBa_K194003</a> | Registry id: <a href="http://partsregistry.org/Part:BBa_K194003" target="_blank">BBa_K194003</a> | ||
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Revision as of 09:28, 18 October 2009
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Parts submitted to registry cln2 PEST destabilization domain for rapid protein turnover C-terminal domain of Saccharomyces cerevisiae cyclin 2 (CLN2) gene. It has been shown that this region of the protein, which is rich of PEST motifs, leads to a destabilization of the protein. Hence this Tag can be used to increase the turn-over rate of a protein. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues changes the half-life of a GFP from 7h and down to 30 minutes. Registry id: BBa_K194000GFP (a yeast- and FACS-optimized GFP variant) Green Fluorescent Protein (GFP) codonoptimized for yeast. Registry id: BBa_K194001Destabilized GFP for yeast This variant of biobrick BBa_K194001 has been destabilized by fusing biobrick BBa_K194000. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes. Registry id: BBa_K194002USER cassette for insertion of USER fragments This biobrick contains a PacI/Nt.BbvCI USER cassette for insertion of PCR fragments using the USER cloning standard. PCR fragments containing uracil is treated with a uracil DNA glycosylase that removes the uracil exposing a predesigned 8bp overhang allowing for cloning without the need for ligase. Registry id: BBa_K194003 |
The Registry of Standard Biological Parts The Registry is a collection of ~3200 genetic parts that can be mixed and matched to build synthetic biology devices and systems. Founded in 2003 at MIT, the Registry is part of the Synthetic Biology community's efforts to make biology easier to engineer. It provides a resource of available genetic parts to iGEM teams and academic labs.
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