Team:Paris/DryLab

Protocols Freezer NoteBook Bibliography Parts WetLab DryLab

Team Collaborations Contacts Acknowledgements Links

Contents 1 DryLab 2 Main 2.1 Questions 2.2 Results

DryLab

Main - Introduction - Delay model - Vesicle model - Fec simulation Main

Main

Questions

Our Message in a Bubble project is fully integrated inside a reflection on communication between bacterias. Among all problems raised during the discussions, we solved 3 of them with modelisation :

• To improve the quality of signal sent, how can we get a synchronisation between the maximum concentration of proteins encapsulated and the maximum quantity of vesicules sent by time units ?

• To obtain the maximum of vesicle, How using the link between Tol-Pal system's disturbing and the creation of vesicles ?

• How can we optimize the quality of the reception i.e getting a robust response with a limited number of messengers sent ?

Results

To create a delay between the maximum concentration of proteins and of the maximum creation of vesicules by time units, we used a transcriptional cascade inside the genetic network thus synchronising protein and vesicule creations.

Plot showing that vesiculation happens only once the protein encapsulated has reached its maximal concentration.

Indeed, the over expression of soluble parts.....

Pierre

Concerning reception, stochastic simulations revealed two possible problems reducing the robustness of our reception system :

• when the amount of messengers received is too weak, the activation does not always occur
• even when it occurs, the activation time can vary.

After trying various solutions in our modeling study, we proposed to introduce an over expression of FecR protein to solve this problem ; simulations showed a good and robust activation in this case.