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Team:IIT Madras/Notebook/Sandbox
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== Intermediates and final constructs planned for the the 2 plasmid locking system == | == Intermediates and final constructs planned for the the 2 plasmid locking system == | ||
| + | == Original design == | ||
| + | |||
| + | In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker). | ||
| + | |||
| + | <html><br></html> | ||
| + | |||
| + | [[Image:2plasmidoriginaldesign.jpg]] | ||
| + | |||
| + | <html><br></html> | ||
| + | <html><br></html> | ||
| + | |||
| + | |||
| + | == Final design == | ||
| + | |||
| + | We decided to remove the extra antibiotic markers from the inserts. Then it looks like this | ||
| + | |||
| + | <html><br></html> | ||
| + | |||
| + | [[Image:2plasmidfinaldesign.jpg]] | ||
| + | |||
| + | <html><br></html> | ||
| + | |||
| + | We had more than one strategy to build the whole construct. | ||
| + | |||
| + | |||
| + | == ''strategy 1'' == | ||
| + | |||
| + | [[Image:2plasmidstrategy1.jpg]] | ||
| + | |||
| + | <html><br></html> | ||
| + | |||
| + | |||
| + | == ''strategy 2'' == | ||
| + | |||
| + | [[Image:2plasmidstrategy2.jpg]] | ||
| + | |||
| + | <html><br></html> | ||
== Intermediates and final constructs planned for the the 3 plasmid locking system == | == Intermediates and final constructs planned for the the 3 plasmid locking system == | ||
Revision as of 17:27, 18 October 2009
Intermediates and final constructs planned for the the 2 plasmid locking system
Original design
In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).
Final design
We decided to remove the extra antibiotic markers from the inserts. Then it looks like this
We had more than one strategy to build the whole construct.
strategy 1
strategy 2
