Team:IIT Madras/Notebook/Sandbox
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== intermediates planned through PCR protocol == | == intermediates planned through PCR protocol == |
Revision as of 17:32, 18 October 2009
Contents |
Intermediates and proposed constructs for the the 2 plasmid locking system
Original design
In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).
Final design
We decided to remove the extra antibiotic markers from the inserts. Then it looks like this
We had more than one strategy to build the whole construct.
strategy 1
strategy 2