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Team:Washington/Notebook/Standard curve
From 2009.igem.org
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====Generating a Standard curve for GFP concentration==== | ====Generating a Standard curve for GFP concentration==== | ||
| - | #Purify GFP-fused protein | + | #Purify GFP-fused protein using traditional purification protocol |
| + | #Measure absorption at 280nm to quantify protein amount | ||
| + | #Serially dilute protein into plate and read fluorescence, excitation 485nm, emission 525nm | ||
| + | #Compare values of protein concentration to fluorescence to create a standard curve allowing for the comparison of fluorescence to protein level | ||
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| + | {{Template:Team:Washington/Templates/Footer}} | ||
Latest revision as of 21:36, 18 October 2009
Generating a Standard curve for GFP concentration
- Purify GFP-fused protein using traditional purification protocol
- Measure absorption at 280nm to quantify protein amount
- Serially dilute protein into plate and read fluorescence, excitation 485nm, emission 525nm
- Compare values of protein concentration to fluorescence to create a standard curve allowing for the comparison of fluorescence to protein level
