Team:Freiburg bioware/cloning1

From 2009.igem.org

(Difference between revisions)
Line 338: Line 338:
ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC
ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC
ATCGGCGACCGGTTA
ATCGGCGACCGGTTA
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MKKIWLALAGLVLAFSASATG
 +
<br><br></p></td></table>
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/1/1b/Freiburg09_DsbA_pMA.txt">DsbA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
<a name="5"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;StrepTag_pMA (iGEM team Freiburg 08)<span class="art-PostHeader"></span> </h3>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 +
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/7/74/Freiburg09_StrepTag_pMA.jpg/600px-Freiburg09_StrepTag_pMA.jpg" name="StrepTag in pMA Vector" width="480" height="480" />
 +
<br><p align="center">StrepTag in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K157012">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGTT
Line 349: Line 379:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
MKKIWLALAGLVLAFSASATG
+
MAGWSHPQFEKTG
-
 
+
<br><br></p></td></table>
<br><br></p></td></table>
<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/1/1b/Freiburg09_DsbA_pMA.txt">DsbA_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/2/22/Freiburg09_StrepTag_pMA.txt">StrepTag_pMA.txt</a></li>
<br>
<br>
<br>
<br>
<br>
<br>
-
 
-
 
-
 

Revision as of 10:30, 19 October 2009

FREiGEM



 FokA_pMA

back to index

FokA in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGA TGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTG ATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGAT GAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAAT TCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACC CGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGA GCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAG AAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

    Commented GenBank file

  • FokA_pMA.txt



  •  Foki_pMA

    back to index

    Foki in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Foki_pMA.txt



  •  Dig_pMA

    back to index

    Dig in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGCAGGTGGCCG CTTATCCCGACCACATCACCAAGTACGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTACAGCGTGATCCACGG CAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCTACACCATCGGCGGCGTGACCCAGGTGGGC GTGAGCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACTT TTGCAGATACGACGAGGACAAGAAGGGCCACTGGGACGCCGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCAACTGGTCCCACCCCC AGTTCGAAAAGACCGGTTAA

    AA sequence of ORF-1:

    MAGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGKSVKVS RYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIGYFCRY DEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNSNW SHPQFEKTG

    Commented GenBank file

  • Dig_pMA.txt



  •  DsbA_pMA

    back to index

    DsbA in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGTTA

    AA sequence of ORF-1:

    MKKIWLALAGLVLAFSASATG

    Commented GenBank file

  • DsbA_pMA.txt



  •  StrepTag_pMA (iGEM team Freiburg 08)

    back to index

    StrepTag in pMA Vector

  • link to part registry
  • nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGTT

    AA sequence of ORF-1:

    MAGWSHPQFEKTG

    Commented GenBank file

  • StrepTag_pMA.txt