"
Team:Tokyo Tech/Iron-oxidizing bacteria
From 2009.igem.org
(→Preparation of 9K medium) |
|||
| Line 1: | Line 1: | ||
| - | Iron-oxidizing bacteria | + | {{Template:Tokyo_Tech_Menu}} |
| + | ==Iron-oxidizing bacteria== | ||
| - | ==Role in the story== | + | ===Role in the story=== |
Why do we need to use Iron-oxidizing bacteria for terraforming of Mars? | Why do we need to use Iron-oxidizing bacteria for terraforming of Mars? | ||
Heterotrophic bacteria cannot live in native Martian environment because there are no organic matters on Mars, however autotrophic bacteria can live without organic matters. | Heterotrophic bacteria cannot live in native Martian environment because there are no organic matters on Mars, however autotrophic bacteria can live without organic matters. | ||
| Line 10: | Line 11: | ||
Accumulation of organic matters provides habitable environment for heterotrophic bacteria (for instance, E.coli). | Accumulation of organic matters provides habitable environment for heterotrophic bacteria (for instance, E.coli). | ||
| - | ==About iron-oxidizing bacteria== | + | ===About iron-oxidizing bacteria=== |
Iron-oxidizing bacterium is a kind of autotrophic bacteria. | Iron-oxidizing bacterium is a kind of autotrophic bacteria. | ||
Iron-oxidizing bacteria reduce NAD+ to NADH by transfer of hydride. | Iron-oxidizing bacteria reduce NAD+ to NADH by transfer of hydride. | ||
In laboratory, iron-oxidizing bacteria are cultured in 9K medium (リンク) which contains Fe(Ⅱ) and no organic matters. | In laboratory, iron-oxidizing bacteria are cultured in 9K medium (リンク) which contains Fe(Ⅱ) and no organic matters. | ||
| - | ==Experimental result== | + | ===Experimental result=== |
We cultured Acidithiobacillus ferrooxidans (iron-oxidizing bacteria). | We cultured Acidithiobacillus ferrooxidans (iron-oxidizing bacteria). | ||
The medium for A.ferrooxidans was blue before cultivation owing to Fe(Ⅱ). | The medium for A.ferrooxidans was blue before cultivation owing to Fe(Ⅱ). | ||
| Line 24: | Line 25: | ||
| - | ==Protocols== | + | ===Protocols=== |
| - | ===Preparation of 9K medium (for Acidithiobacillus ferrooxidans)=== | + | ====Preparation of 9K medium (for Acidithiobacillus ferrooxidans)==== |
*Prepare following A&B solution. | *Prepare following A&B solution. | ||
| Line 33: | Line 34: | ||
*A solution can be stored for 2 weeks. | *A solution can be stored for 2 weeks. | ||
| - | ===Culturing method=== | + | ====Culturing method==== |
Culture in 9K medium at 30℃ with vigorous shaking. | Culture in 9K medium at 30℃ with vigorous shaking. | ||
Use the microscope when confirm the growth. | Use the microscope when confirm the growth. | ||
Revision as of 13:04, 19 October 2009
| Main | Team | Terraforming | Experiments | [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Tokyo_Tech Parts] | Safety |
Contents |
Iron-oxidizing bacteria
Role in the story
Why do we need to use Iron-oxidizing bacteria for terraforming of Mars? Heterotrophic bacteria cannot live in native Martian environment because there are no organic matters on Mars, however autotrophic bacteria can live without organic matters. Iron-oxidizing bacteria is autotrophic bacteria. Iron-oxidizing bacteria gets energy by oxidizing Fe(Ⅱ). To grow on Mars, iron-oxidizing bacteria require only energy injection of us because of composition of the Martian surface, crust and atmosphere. (リンク) We propose culturing iron-oxidizing bacteria on Mars and producing organic matters. Accumulation of organic matters provides habitable environment for heterotrophic bacteria (for instance, E.coli).
About iron-oxidizing bacteria
Iron-oxidizing bacterium is a kind of autotrophic bacteria. Iron-oxidizing bacteria reduce NAD+ to NADH by transfer of hydride. In laboratory, iron-oxidizing bacteria are cultured in 9K medium (リンク) which contains Fe(Ⅱ) and no organic matters.
Experimental result
We cultured Acidithiobacillus ferrooxidans (iron-oxidizing bacteria). The medium for A.ferrooxidans was blue before cultivation owing to Fe(Ⅱ). The presence of A.ferrooxidans changed the medium into brown one after culturing for two days. This change was observed owing to the production of Fe(OH)3. We confirmed the growth by using microscope of 200 magnifications.
Protocols
Preparation of 9K medium (for Acidithiobacillus ferrooxidans)
- Prepare following A&B solution.
- Sterilize A solution by autoclaving.
- Sterilize B solution by using filter.
- Mix solution A and B before use.
- A solution can be stored for 2 weeks.
Culturing method
Culture in 9K medium at 30℃ with vigorous shaking. Use the microscope when confirm the growth.
