Team:Paris/Protocols

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== Protocols ==
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==Main==  
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<center> [[team:Paris/Protocols#bottom|Main]] - [[Team:Paris/ProtocolsA#bottom | Microscope Protocol]] - [[Team:Paris/ProtocolsB#bottom | Adapted Protocols]] - [[Team:Paris/Protocols_Culture#bottom | Culture protocols]] - [[Team:Paris/ProtocolsMB#bottom | Molecular Biology]]</center>
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Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a mix with previous Paris iGEM team .
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<center> [[team:Paris/Protocols#Protocols|Main]] - [[Team:Paris/ProtocolsA#Protocols | Microscope Protocol]] - [[Team:Paris/ProtocolsB#Protocols | Adapted Protocols]] - [[Team:Paris/Protocols_Culture#Protocols | Culture protocols]] - [[Team:Paris/ProtocolsMB#Protocols | Molecular Biology]]</center>
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<center> '''Main'''</center>
<center> '''Main'''</center>
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Protocols#Protocols"> Main </a>|
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Protocols#bottom"> Main </a>|
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<a class="menu_sub" href="https://2009.igem.org/Team:Paris/ProtocolsA#Protocols"> Microscope Protocols</a>|
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<a class="menu_sub" href="https://2009.igem.org/Team:Paris/ProtocolsA#bottom"> Microscope Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsB#Protocols"> Adapted Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsB#bottom"> Adapted Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Protocols_Culture#Protocols"> Culture Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Protocols_Culture#bottom"> Culture Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsMB#Protocols"> Molecular biology</a>
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsMB#bottom"> Molecular biology</a>
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==Summary==
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===1. Microscopy===
===1. Microscopy===
* [[Team:Paris/ProtocolsA#Protocols suggested | Protocols suggested]]
* [[Team:Paris/ProtocolsA#Protocols suggested | Protocols suggested]]

Revision as of 15:00, 19 October 2009

iGEM > Paris > Protocols > Main


Main

Main - Microscope Protocol - Adapted Protocols - Culture protocols - Molecular Biology
Main
===1. Microscopy=== * [[Team:Paris/ProtocolsA#Protocols suggested | Protocols suggested]] *[http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method (pdf)] ===2. Adapted Protocols=== * DNA extraction * [[Team:Paris/ProtocolsB#Adaptation of PureYield™ Plasmid Miniprep System (Promega) |Mini prep ]] * Gel/PCR Purification flefevre : si vous avez rien a dire ou pas le temps, il faut mieux supprimer des categories plutot que d avoir x pages pas créer ===3. Culture protocols=== * Over Night *[[Team:Paris/Protocols_Culture#Bacterial transformation | Bacterial transformation]] * [[Team:Paris/Protocols_Culture#Competent bacteria | Competent bacteria]] **[[team:Paris/Protocols_Culture#CaCl2a|CaCl2 (iGEM Paris2007)]] **[[team:Paris/Protocols_Culture#CaCl2b|CaCl2 (2nd Protocol)]] **[[team:Paris/Protocols_Culture#RbCl|RbCl]] * [[team:Paris/Protocols_Culture#Glycerol stock|Glycerol stock]] *[[team:Paris/Protocols_Culture#Ferric citrate growth medium|Ferric citrate growth medium]] ===4. Molecular biology=== *[[Team:Paris/ProtocolsMB#PCR with Quick load Taq2x Master Mix|PCR]] *[[Team:Paris/ProtocolsMB#PCR colonies|PCR colony]] * Gels * Digestion * Ligation