Team:Paris/ProtocolsMB

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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Protocols#top | Protocols]] > [[Team:Paris/ProtocolsMB#bottom | Molecular Biology]]
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{{Template:Paris2009}}
{{Template:Paris2009_menu}}
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== Protocols ==
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<center> [[team:Paris/Protocols#Protocols|Main]] - [[Team:Paris/ProtocolsA#Protocols | Microscopy Protocol]] - [[Team:Paris/ProtocolsB#Protocols | Adapted Protocols]] - [[Team:Paris/Protocols_Culture#Protocols | Culture]] - [[Team:Paris/ProtocolsMB#Protocols | Molecular Biology]]</center>
 
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==PCR with Quick load Taq2x Master Mix==
<html>
<html>
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<style type="text/css">
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#left-side {
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    position: absolute;
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    height: 23px;
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    width: 30px;
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    top: 0px;
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    left: 40px;
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    margin-top:10px;
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    padding-top: 7px;
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    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
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    z-index:4;
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}
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#middle-side {
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    height: 25px;
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    width: 560px;
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    position: absolute;
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    top: 0px;
 +
    left: 60px;
 +
    margin-top:10px;
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    padding-top: 5px;
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    background: #dadada;
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    z-index:5;
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}
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#right-side {
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    position: absolute;
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    height: 23px;
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    width: 30px;
 +
    margin-top:10px;
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    padding-top: 7px;
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    top: 0px;
 +
    left: 600px;
 +
    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
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    z-index:4;
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}
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a.menu_sub {
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    padding-left: 7px;
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    padding-right: 7px;
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}
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a.menu_sub_active {
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    padding-left: 7px;
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    padding-right: 7px;
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    color:#b0310e;
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    font-weight:bold;
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}
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</style>
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<div id="left-side"></div>
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<div id="middle-side"><center>
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Protocols#bottom"> Main </a>|
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<a class="menu_sub" href="https://2009.igem.org/Team:Paris/ProtocolsA#bottom"> Microscope Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsB#bottom"> Adapted Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Protocols_Culture#bottom"> Culture Protocols</a>|
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/ProtocolsMB#bottom"> Molecular biology</a>
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</center>
</div>
</div>
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<div id="paris_content_boxtop">
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</div>
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<div id="right-side"></div>
-
<div id="paris_content">
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</html>
</html>
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*Mix for 50µl final:
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**14µL H2O
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**25µL Taq Quick load Master Mix 2X
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**5µL Oligo Fw (0,2µM)
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**5µL Oligo Rw (0,2µM)
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**1µL Matrice (DNA)

Latest revision as of 15:12, 19 October 2009

iGEM > Paris > Protocols > Molecular Biology


PCR with Quick load Taq2x Master Mix

  • Mix for 50µl final:
    • 14µL H2O
    • 25µL Taq Quick load Master Mix 2X
    • 5µL Oligo Fw (0,2µM)
    • 5µL Oligo Rw (0,2µM)
    • 1µL Matrice (DNA)

Retrieved from "http://2009.igem.org/Team:Paris/ProtocolsMB"