Team:Imperial College London/M2/EncapsulationRationale

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=[[Image:II09_Thumb_m2.png|40px]]<font size='5'><b>Module 2 - Encapsulsation Overview</b></font>=
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=[[Image:II09_Thumb_m2.png|40px]]<font size='5'><b>Module 2 - Acid Resistance Overview</b></font>=
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=Encapsulation Rationale=
 
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In nature, encapsulation pathways such as spore formation, aliginate biosynthesis and colanic acid production all share one common feature: they require a large number of genes. For this reason, we decided that the best way encapsulate our chassis was via the modulation of an existing pathway.
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<i>E.coli</i> naturally produces a harmless acid resistant polymer known as colanic acid. Colanic acid is a polymer of glucose, galactose and glucuronic acid. By tapping into the pathway that initiates colanic acid biosynthesis, we can turn on its production via the modulation of a gene called RcsB.
 
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In nature, colanic acid acts as a binding agent between individual cells over which a biofilm can be formed. While colanic acid itself is harmless, biofilm formation is associated with the production of a number of virulence factors. To prevent biofilm formation from occuring, we have tapped into a second pathway such that our cells become locked into colanic acid production. The gene responsible for preventing biofilm formation is a transcription factor called YgiV.
 
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In nature, colanic acid is associated with but not attached to the cell surface. To facilitate whole cell encapsulation, we have modified a third pathway to fix the colanic acid to the surface of the cell. This involves an enzyme called Rfal.
 
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<html><a href="https://2009.igem.org/Team:Imperial_College_London/M2/genes"><img width=90px src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png" align="left"></a></html><br><br> &nbsp; About RcsB, YgiV and Rfal.
 
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  <center><b>Module 2 - Encapsulation</b></center>  
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===Module 2 - Encapsulation===
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<html><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/M2/FreezeDrying"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png"></a><a
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href="https://2009.igem.org/Team:Imperial_College_London/M2/Genetic"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png"></a><a
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href="https://2009.igem.org/Team:Imperial_College_London/M2/Wetlab"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png"></a><html><a
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href="https://2009.igem.org/Team:Imperial_College_London/M2/Modelling"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png"></a><center></html>
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<html><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/M2/FreezeDrying"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_freezedrying.png"></a><a
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href="https://2009.igem.org/Team:Imperial_College_London/M2/Genetic"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_geneticcircuit1.png"></a><a
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href="https://2009.igem.org/Team:Imperial_College_London/M2/Wetlab"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Wetlabmainimage9.png"></a><html><a
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href="https://2009.igem.org/Team:Imperial_College_London/M2/Modelling"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage6.png"></a><center></html>
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<html><table border="0" style="background-color:transparent;" width="100%">
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<td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2/Genetic"><b>Genetic Circuit</b></a></center></td>
<td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2/Genetic"><b>Genetic Circuit</b></a></center></td>
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<td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/M2/Wetlab"><b>WetLab</b></a></center></td>
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<td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/M2/Wetlab"><b>Wet Lab</b></a></center></td>
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<html><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2#Module_2_Contents"><img width=150px src="https://static.igem.org/mediawiki/2009/1/10/II09_TourArrow.png"></a>
<html><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2#Module_2_Contents"><img width=150px src="https://static.igem.org/mediawiki/2009/1/10/II09_TourArrow.png"></a>

Latest revision as of 21:44, 19 October 2009

II09 Thumb m2.pngModule 2 - Acid Resistance Overview

II09 TimelineM2.png





Module 2 - Encapsulation

Module 2 Overview
Freeze Drying
Genetic Circuit
Wet Lab
Modelling

Mr. Gene   Geneart   Clontech   Giant Microbes

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