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Team:Nevada/Team
From 2009.igem.org
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-Primer design for adding Biobrick restriction sites for formation of Biobrick parts | -Primer design for adding Biobrick restriction sites for formation of Biobrick parts | ||
| - | [[Janice Cho]] | + | [[Janice Cho]]<BR> |
| - | - Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000. | + | -Formulated the original idea of using cinnamaldehyde as a mosquito larvicide. <BR> |
| + | -Performed initial research on the use of cinnamaldehyde. | ||
| + | - Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000.<BR> | ||
- Helped conduct a three-way ligation to create final construct with the parts: BBa_K262000 and BBa_K262002. | - Helped conduct a three-way ligation to create final construct with the parts: BBa_K262000 and BBa_K262002. | ||
| - | [[Leigh Armijo]] | + | [[Leigh Armijo]]<BR> |
| - | - Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000. | + | - Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000. <BR> |
- Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014. | - Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014. | ||
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[[Tony Moschetti]] | [[Tony Moschetti]] | ||
<BR> | <BR> | ||
| - | -Made Biobrick part containing cinnamoyl-CoA reductase ( | + | -Topo-cloned cinnamoyl-CoA reductase (gene 3)for preparation of a Biobrick part. |
| + | <BR> | ||
| + | -Made Biobrick part containing cinnamoyl-CoA reductase (gene 3) and double terminator (BBa_K262002) | ||
<BR> | <BR> | ||
-Transformed 4-coumarate-CoA ligase (gene 2) and cinnamoyl-CoA reductase (gene 3 - BBa_K262001) into Biobrick backbones. | -Transformed 4-coumarate-CoA ligase (gene 2) and cinnamoyl-CoA reductase (gene 3 - BBa_K262001) into Biobrick backbones. | ||
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[[Chris Martinez]] <BR> | [[Chris Martinez]] <BR> | ||
| + | -Made db3.1 and NEB10 competent cells. <BR> | ||
-Overall goal was to upregulate the cinnamaldehyde pathway in plants ''Wolffia'' and various ''Lemna'' species <BR> | -Overall goal was to upregulate the cinnamaldehyde pathway in plants ''Wolffia'' and various ''Lemna'' species <BR> | ||
-Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector <BR> | -Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector <BR> | ||
-Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants ''Wolffia'' and ''Lemna'' species | -Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants ''Wolffia'' and ''Lemna'' species | ||
| + | |||
| + | [[Joey Khwaja]]<BR> | ||
| + | -Made db3.1 and NEB10 competent cells. <BR> | ||
| + | -Researched into making a Biobrick plant part.<BR> | ||
| + | -Designed the iGEM Nevada Team shirts. | ||
== '''Where we're from''' == | == '''Where we're from''' == | ||
Revision as of 00:46, 20 October 2009
Contents |
Who we are
Advisors
- Advisor 1: Dr. Christie Howard
- Advisor 2: Dr. Patricia Ellison
- Advisor 3: Dr. Dave Shintani
- Grad Student 1: Chad Sanada
Undergrads
- Student 1: Nick Tschernia
- Student 2: Tony Moschetti
- Student 3: Joey Khwaja
- Student 4: Chris Martinez
- Student 5: Janice Cho
- Student 6: Leigh Armijo
- Student 7: Sheena Jones
- Student 8: Tyler Peterson
- Student 9: J Malikowski
- Student 10: Alex Dussaq
What we did
Nick Tschernia
-Entered the Nevada Team into the iGEM Competition a week before the submission date.
-Organized weekly meetings for project brainstorming and timeline.
-Recruited the iGEM Team through a highly selective process (whoever was left at the end of the brainstorming sessions).
-Organized the source plate distribution and transformation of the basic parts.
-Sequence analysis of the Biobrick parts formed.
-Primer design for adding Biobrick restriction sites for formation of Biobrick parts
Janice Cho
-Formulated the original idea of using cinnamaldehyde as a mosquito larvicide.
-Performed initial research on the use of cinnamaldehyde.
- Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000.
- Helped conduct a three-way ligation to create final construct with the parts: BBa_K262000 and BBa_K262002.
Leigh Armijo
- Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000.
- Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014.
Sheena Jones
-In charge of maintaining and propagating Wolffia and Lemna plant species.
-Generated callus culture in preparation for gene transformation.
Tony Moschetti
-Topo-cloned cinnamoyl-CoA reductase (gene 3)for preparation of a Biobrick part.
-Made Biobrick part containing cinnamoyl-CoA reductase (gene 3) and double terminator (BBa_K262002)
-Transformed 4-coumarate-CoA ligase (gene 2) and cinnamoyl-CoA reductase (gene 3 - BBa_K262001) into Biobrick backbones.
J Malikowski
-Induced expression in non-biobrick expression vector containing 4-coumarate-CoA ligase (gene 2). Detection of product formation at 311 nm. Further studies will be conducted in the near future.
-Helped to troubleshoot minipreps and other protocols. Resident expert on minipreps.
-Trained Tyler to be an excellent Molecular Biologist.
-Advisory role at lab meetings (How does the Harper lab do it?)
Tyler Peterson
-Major player in media preparation, agarose gels, stock solutions
-Helped with minipreps and E. coli transformation.
Chris Martinez
-Made db3.1 and NEB10 competent cells.
-Overall goal was to upregulate the cinnamaldehyde pathway in plants Wolffia and various Lemna species
-Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector
-Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants Wolffia and Lemna species
Joey Khwaja
-Made db3.1 and NEB10 competent cells.
-Researched into making a Biobrick plant part.
-Designed the iGEM Nevada Team shirts.
Where we're from
