Team:LCG-UNAM-Mexico/AbrahamJurnal
From 2009.igem.org
(Difference between revisions)
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=='''Abraham's lab journal'''== | =='''Abraham's lab journal'''== | ||
- | Protocols | + | '''Protocols''' |
Colony PCR<br> | Colony PCR<br> | ||
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Objectives: | Objectives: | ||
- | The final aim is to earn all the following devices and | + | The final aim is to earn all the following devices and them all together into the plasmid we are suggesting, that is extracted from P4 |
- | [[Image:Sistema. | + | [[Image:Sistema.JPG]] |
+ | |||
+ | I learned to obtain the plasmid send by iGEM in the 2009 kit plates, and to transform it. | ||
+ | |||
+ | '''July 10th''' | ||
+ | ---- | ||
- | |||
- | |||
We transformed the plasmids coming in the 2009 kit plates, | We transformed the plasmids coming in the 2009 kit plates, | ||
BBa_I715038 ready to use T7 RNA polymerase inducible with IPTG | BBa_I715038 ready to use T7 RNA polymerase inducible with IPTG | ||
Line 127: | Line 130: | ||
The final result is following: | The final result is following: | ||
The pink sequence represents the 5’UTR, the blue one is where the RBS is supposed to be, the common part is just the 5’ of the gene. | The pink sequence represents the 5’UTR, the blue one is where the RBS is supposed to be, the common part is just the 5’ of the gene. | ||
+ | |||
GGGTGGCCTTTATGATTATCATTTAGCACGAAACCAAAGGAGGGCATTATGCTCGTAAGTGACATTGAGG | GGGTGGCCTTTATGATTATCATTTAGCACGAAACCAAAGGAGGGCATTATGCTCGTAAGTGACATTGAGG | ||
- | + | t3 DNA polymerase | |
AAACGAAACCTAAAGGAGATTAACATTATGGCTAAGAAGATTTTCACCTCTGCGCTGGGTACCGCTGAACCTTACGCTTACAT | AAACGAAACCTAAAGGAGATTAACATTATGGCTAAGAAGATTTTCACCTCTGCGCTGGGTACCGCTGAACCTTACGCTTACAT | ||
- | + | t7 gp2.5 ssDNA Binding Prot | |
Their reverse complement sequences are: | Their reverse complement sequences are: | ||
- | |||
- | Form two possible secondary structures of -10 and -11 kcal/mol. | + | *CCTCAATGTCACTTACGAGCATAATGCCCTCCTTTGGTTTCGTGCTAAATGATAATCATAAAGGCCACCC. |
- | ATGTAAGCGTAAGGTTCAGCGGTACCCAGCGCAGAGGTGAAAATCTTCTTAGCCATAATGTTAATCTCCTTTAGGTTTCGTTT | + | Form two possible secondary structures of -10 and -11 kcal/mol. |
- | Three possible secondary structures between -13.3 and 14.3 kcal/mol | + | |
+ | *ATGTAAGCGTAAGGTTCAGCGGTACCCAGCGCAGAGGTGAAAATCTTCTTAGCCATAATGTTAATCTCCTTTAGGTTTCGTTT | ||
+ | Three possible secondary structures between -13.3 and 14.3 kcal/mol | ||
Revision as of 01:30, 20 October 2009