Team:Uppsala-Sweden/Modelling
From 2009.igem.org
(Difference between revisions)
m (→Modelling of ethanol production) |
m (→Modelling of ethanol production) |
||
Line 17: | Line 17: | ||
[[Image:ek_1_2.png]] | [[Image:ek_1_2.png]] | ||
+ | |||
+ | Having the Km and Kcat values gives e0 from level of product formation. | ||
[[Image:ek_3.png]] | [[Image:ek_3.png]] | ||
+ | |||
+ | Having e0 and letting kt go towards zero, (kt here represents the rate at which the substrate "disappears" into the Krebbs cycle), gives ks | ||
+ | |||
+ | [[Image:ek_4.png]] | ||
+ | |||
Revision as of 09:07, 20 October 2009
Modelling of ethanol production
For our modell we have some basic assumptions that are
1. Co-evolution of the of the ethanol producing pathway, making km and kcat being highly similar for pdc as well as ADH
2. Assuming quasi steady state (Michaelis Menten Chinetics
e0=e+es
Having the Km and Kcat values gives e0 from level of product formation.
Having e0 and letting kt go towards zero, (kt here represents the rate at which the substrate "disappears" into the Krebbs cycle), gives ks