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Team:HKUST/Protocols
From 2009.igem.org
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| - | <li><a href="https://2009.igem.org/Team:HKUST/ | + | <li><a href="https://2009.igem.org/Team:HKUST/OdorantSensing">Odorant Sensing</a></li> |
| - | <li><a href="https://2009.igem.org/Team:HKUST/ | + | <li><a href="https://2009.igem.org/Team:HKUST/AttractantProduction">Attractant Production</a></li> |
<li><a href="https://2009.igem.org/Team:HKUST/ToxinProduction">Toxin Production</a></li> | <li><a href="https://2009.igem.org/Team:HKUST/ToxinProduction">Toxin Production</a></li> | ||
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<ul> | <ul> | ||
<li><a href="https://2009.igem.org/Team:Gallery">Gallery</a></li> | <li><a href="https://2009.igem.org/Team:Gallery">Gallery</a></li> | ||
| - | <li><a href="https://2009.igem.org/Team: | + | <li><a href="https://2009.igem.org/Team:Biosafety">Biosafety</a></li> |
<li><a href="https://2009.igem.org/Team:Acknowledgement">Acknowledgement</a></li> | <li><a href="https://2009.igem.org/Team:Acknowledgement">Acknowledgement</a></li> | ||
</ul> | </ul> | ||
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</div> | </div> | ||
<div id="rightxx"> | <div id="rightxx"> | ||
| - | <div class=" | + | <div class="contentlist"> <h3>a</h3> |
</div> | </div> | ||
<div class="contentxx"> | <div class="contentxx"> | ||
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/Western_blotting"> Western blotting</a></li> | <li><a href="https://2009.igem.org/Team:HKUST/Protocols/Western_blotting"> Western blotting</a></li> | ||
<li><a href="https://2009.igem.org/Team:HKUST/Protocols/GFP_testing"> GFP testing</a></li> | <li><a href="https://2009.igem.org/Team:HKUST/Protocols/GFP_testing"> GFP testing</a></li> | ||
| - | + | ||
</ul> | </ul> | ||
Latest revision as of 09:53, 20 October 2009
a
- Agarose gel preparation and gel electrophoresis
- PCR (Taq, Vent)
- PCR product clean-up
- Enzyme digestion (vector, insert)
- Plasmid DNA extraction
- Gel cutting
- Gel extraction
- Ethanol precipitation
- Ligation
- Transformation to E.coli
- Transformation to yeast
- Cell lysis
- Yeast genomic DNA extraction
- Western blotting
- GFP testing
iGEM 2009
