Team:HKUST/Protocols/gel cut

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(New page: 6. Cutting bands on the gel Purpose: To extract specific DNA fragment. Procedure: a.Visualize the bands under UV light. Use long-wavelength UV to minimize damage to the DNA. b...)
 
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6. Cutting bands on the gel
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  Purpose: To extract specific DNA fragment.
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<head>
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  Procedure:  
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<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" />
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   a.Visualize the bands under UV light. Use long-wavelength UV to minimize damage to the DNA.
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<link href="http://igem2009hkust.fileave.com/wiki/template/12092009/style.css " rel="stylesheet" type="text/css" />
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   b.Cut the band with a clean razor blade.
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<title>Salt and Soap template</title>
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   c.Turn the gel slice on its side to trim off extra agarose. Place the gel in a microcentrifuge tube.
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</head>
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  Tips:
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  •EB staining is needed for higher resolution under UV light.
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  Safety Tips:  
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  •Permission is needed from technician before doing this! Follow the safety instruction in the room.
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  •When using UV light, protect your skin by wearing safety goggles or a face shield, gloves, and a lab coat.
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<div class="contentlist"> <h3>a</h3>
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<p>Cutting bands on the gel</p>
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<p> Purpose: To extract specific DNA fragment.</p>
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<p>Procedure: </p>
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   a.Visualize the bands under UV light. Use long-wavelength UV to minimize damage to the DNA. <br>
 +
   b.Cut the band with a clean razor blade.<br>
 +
   c.Turn the gel slice on its side to trim off extra agarose. Place the gel in a microcentrifuge tube.</p>
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<p> Tips: </p>
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EB staining is needed for higher resolution under UV light.
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<br><br>
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<p> Safety tips: </p>
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1. ermission is needed from technician before doing this! Follow the safety instruction in the room.<br><br>
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2.When using UV light, protect your skin by wearing safety goggles or a face shield, gloves, and a lab coat. <br><br>
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<ul>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/Agarose_gel" >Agarose gel preparation and gel
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electrophoresis</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/PCR">PCR (Taq, Vent)</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/PCR_cleanup">PCR product clean-up</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/Enzyme_digestion"> Enzyme digestion (vector, insert)</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/plasmid_extraction"> Plasmid DNA extraction</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/gel_cut"> Gel cutting</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/gel_extraction"> Gel extraction</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/Ethanol_precipitation"> Ethanol precipitation</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/Ligation"> Ligation</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/Transformation_to_Ecoli"> Transformation to E.coli</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/Transformation_to_yeast">Transformation to
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yeast</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/Cell_lysis"> Cell lysis</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/Yeast_genomic_DNA_extraction"> Yeast genomic DNA
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extraction</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/Western_blotting"> Western blotting</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/Protocols/GFP_testing"> GFP testing</a></li>
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Latest revision as of 09:55, 20 October 2009

Salt and Soap template

a

Cutting bands on the gel

Purpose: To extract specific DNA fragment.

Procedure:

a.Visualize the bands under UV light. Use long-wavelength UV to minimize damage to the DNA.
b.Cut the band with a clean razor blade.
c.Turn the gel slice on its side to trim off extra agarose. Place the gel in a microcentrifuge tube.

Tips:

EB staining is needed for higher resolution under UV light.

Safety tips:

1. ermission is needed from technician before doing this! Follow the safety instruction in the room.

2.When using UV light, protect your skin by wearing safety goggles or a face shield, gloves, and a lab coat.

HKUST

Retrieved from "http://2009.igem.org/Team:HKUST/Protocols/gel_cut"