Team:HKUST/Protocols/GFP testing

From 2009.igem.org

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<li><a href="https://2009.igem.org/Team:HKUST/OdorantSensoring">Odorant Sensoring</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/OdorantSensing">Odorant Sensing</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/AttranctantProduction">Attranctant Production</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/AttractantProduction">Attractant Production</a></li>
<li><a href="https://2009.igem.org/Team:HKUST/ToxinProduction">Toxin Production</a></li>
<li><a href="https://2009.igem.org/Team:HKUST/ToxinProduction">Toxin Production</a></li>
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<li><a href="https://2009.igem.org/Team:Gallery">Gallery</a></li>
<li><a href="https://2009.igem.org/Team:Gallery">Gallery</a></li>
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<li><a href="https://2009.igem.org/Team:Consolidation">Consolidation</a></li>
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<li><a href="https://2009.igem.org/Team:Biosafety">Biosafety</a></li>
<li><a href="https://2009.igem.org/Team:Acknowledgement">Acknowledgement</a></li>
<li><a href="https://2009.igem.org/Team:Acknowledgement">Acknowledgement</a></li>
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Latest revision as of 09:57, 20 October 2009

Salt and Soap template

a

Fluorescence microscopy visualization

Procedure:

1. Grow yeast transformed cells containing appropriate plasmids in 5 ml SCM minus His & glucose at 30ºC overnight.
2. Add galactose (to a conc.of 2%) at a cell density corresponding to an OD600 of a 10-fold dilution reaching 0.8.
3. Continue growing cells at 30ºC for 30min to 1.5hr.
4. At time 30min, 60min, 90min, use 1ml of cells, spin down and resuspend cells in 10μl ddH2O.
5. Use 2μl cells and visualize the cells in a fluorescency microscopy.

HKUST

Retrieved from "http://2009.igem.org/Team:HKUST/Protocols/GFP_testing"