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September/17 September 2009
From 2009.igem.org
(Difference between revisions)
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Latest revision as of 11:10, 20 October 2009
1.colony check
sample no. of colony 45 0 46 0 49 0
2.mini prep
result of nano drop
sample conc. 17 14.1 ng/uL 20-A 22.8 20-B 24.3 27 16.7 28 23.3 30 26.6 35 19.4 37 19.2 48-A 31.5 48-B 20.4
3.digestion
K204050
| Vector | Insert | ||
|---|---|---|---|
| 1-12A | 8 | no.17 | 12 |
| EcoRI | 1 | EcoRI | 1 |
| XbaI | 1 | SpeI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 8 | dH2O | 4 |
| total | 20uL | total | 20uL |
↓
37 degree 3hr
gel electrophoresis
gelcut
4.transformation
sample No. 29 , 38
