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September/22 September 2009
From 2009.igem.org
(Difference between revisions)
(New page: 1. colony check sample no no. of colony 36 5 37 0 38 1 29 0 <br> 2.min prep<br> sample no conc. 3-7G ...) |
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22 , 25 , 40 , 47 | 22 , 25 , 40 , 47 | ||
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| + | [https://2009.igem.org/Team:Osaka/NOTES back to NOTES] | ||
Latest revision as of 11:11, 20 October 2009
1. colony check
sample no no. of colony 36 5 37 0 38 1 29 0
2.min prep
sample no conc.
3-7G 94.8 ng/uL
2-3I 114
1-8K 144
2-18F 120
136
46 77
2-9A 132
3-7C 149
27B 104
29A 7.9
46 65
3.transfromation
sample no. X1-A , X2-A , X3-A 1-12M
4.digestion
K204028
| Vector | Insert | ||
|---|---|---|---|
| 2-18H | 3 | 2-16N | 8 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 13 | dH2O | 8 |
| total | 20uL | total | 20uL |
K204030
| Vector | Insert | ||
|---|---|---|---|
| 2-18F | 2 | 1-19B | 5 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 14 | dH2O | 11 |
| total | 20uL | total | 20uL |
↓
37°C, 2hr
↓
gel electrophoresis
gel cut
purification
↓
ligation O/N
5.transformation
sample no. 22 , 25 , 40 , 47
